A3352

アシルコエンザイムA合成酵素 Pseudomonassp. （シュードモナス）由来

≥2 units/mg protein

• 別名: 酸:CoAリガーゼ(AMP生成)
• CAS番号: 9013-18-7
• Enzyme Commission number: 6.2.1.3 (BRENDA, IUBMB)
• EC Number: 232-747-5
• MDL番号: MFCD00130343
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• フォーム: powder
• 品質水準: 200
• 比活性: ≥2 units/mg protein
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

Acyl-coenzyme A synthetase belongs to adenylate-forming enzymes superfamily.[1] It has a conserved adenosine triphosphate/adenosine monophosphate (ATP/AMP) binding motif.[2]

アプリケーション

Acyl-coenzyme A (coA) synthetase from Pseudomonas sp. has been used:

• as ligase in the synthesis of mevalonate derivatives of adenosine triphosphate (ATP)[3]
• in in vitro fatty acylation assays[4]
• in the synthesis of (¹⁴C)Vernoloyl-CoA (Va-CoA),(3) bisphosphonates derivatives of ATP(4) and (³H)Palmitate-CoA[5]

Acyl-coenzyme A Synthetase may be used to study fatty acid metabolism and lipid metabolism. It has been used to study its interaction with fatty acid transport proteins, which has been found to be involved in the efficient cellular uptake of long-chain fatty acids in adipocytes [6].

生物化学的／生理学的作用

Acyl coenzyme A synthetase proteins are involved in regulating and facilitating long-chain fatty acid transport in mammalian cells [6].

Acyl-coenzyme A (CoA) synthetase (ACS) enzyme catalyzes a two-step thioesterification reaction involving the conversion of free fatty acids (FAs) to CoA esters. The substrate for ACS varies from two carbon to 26 carbon FAs. It is involved in the activation of FAs for lipid metabolism and enables FA to participate in various cellular metabolic pathways.[2]

単位の定義

1ユニットは、CoAの存在下で、pH 8.1、25°C、1分間に、1.0 μmolのAMPおよびオレオイルCoAをATPおよびオレイン酸から生成する酵素量です。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)