A0856

抗ATG5 (N-末端) ウサギ宿主抗体

affinity isolated antibody, PBS solution

• 別名: Anti-APG5, Anti-APG5L, Anti-ASP, Anti-ATG5 autophagy related 5 homolog (S. cerevisiae), Anti-Apoptosis-specific protein, Anti-Autophagy protein 5-like
• MDL番号: MFCD09753847
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: PBS solution
• 分子量: antigen ~56 kDa (Atg5-Atg12 complex)
• 化学種の反応性: mouse, human, rat
• 包装: antibody small pack of 25 μL
• テクニック: immunofluorescence: suitable; western blot: 0.5-1 μg/mL using whole extracts of human K562, rat NRK, and mouse 3T3 cells
• UniProtアクセッション番号: Q9H1Y0
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ATG5(9474) ; mouse ... Atg5(11793) ; rat ... Atg5(365601)

詳細

特異性

Rabbit anti-ATG5 (N-terminal) recognizes human, rat, and mouse Atg5-Atg12 complex.

免疫原

ヒトATG5のアミノ酸2-15に相当する合成ペプチド(C末端システイン残基)のKLH結合体。この配列はラットおよびマウスにおいて同一です。

アプリケーション

Anti-ATG5 (N-terminal) antibody has been used:

• in immunoblotting
• in immunofluorescence
• in western blotting

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Rabbit anti-ATG5 (N-terminal specific) was used for western blot analysis to detect the level of ATG5 in SK-N-MC cells after transduction with an adenovirus expressing a shRNA specific to ATG5.

Rabbit anti-ATG5 has been used for immunofluorescence and chemiluminescent western blot analyses.

生物化学的／生理学的作用

Atg5 (Apg5) is a 32 kDa protein that is required for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. The Atg12-Atg5-Atg16 complex thus formed, localizes to autophagosome precursors and regulates autophagosome formation. Studies have reported that Atg5 fragment that is formed by calpain cleavage has pro-apoptotic properties.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)