After dispensing, briefly flush the vial headspace with dry nitrogen or argon and cap immediately. The headspace may be cleared by directing the inert gas across the opening of the vial or bottle. Alternatively, handling in an inert-gas enclosure, such as a glove box or Aldrich® AtmosBag (Z530212), is also suitable. Prepared 1 M solutions are stable for 3 to 6 months when stored refrigerated at 2 -8 °C and up to 12 months at - 20 °C, when stored in single use aliquots. Avoid repeated freeze/thaw cycles. While there is no specific guidance on clearing the headspace for prepared solutions, this additional measure would further ensure stability.
85558
スペルミジン
BioUltra, Molecular Biology, ≥99.5% (GC)
別名:
SPD, 1,8-ジアミノ-4-アザオクタン, N-(3-アミノプロピル)-1,4-ジアミノブタン
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| サイズ/SKU | 在庫状況 | 単価 |
|---|---|---|
1 g | 在庫あり出荷可能 | ￥25,800 |
5 g | カートの在庫状況を確認する | ￥87,200 |
この商品について
化学式:
NH2(CH2)3NH(CH2)4NH2
CAS番号:
分子量:
145.25
UNSPSC Code:
12352116
NACRES:
NA.26
PubChem Substance ID:
EC Number:
204-689-0
Beilstein/REAXYS Number:
1698591
MDL number:
Assay:
≥99.5% (GC)
grade
Molecular Biology
Quality Segment
product line
BioUltra
assay
≥99.5% (GC)
storage condition
(Tightly closed. Keep locked up or in an area accessible only to qualified or authorizedpersons.)
technique(s)
ChIP: suitable, buffer exchange: suitable
impurities
DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
refractive index
n20/D 1.479 (lit.)
pH
12.0-13.5 (25 °C, 1 M in H2O)
solubility
H2O: 1 M at 20 °C, clear, colorless
density
0.925 g/mL at 25 °C (lit.)
anion traces
chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg
cation traces
Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
λ
1 M in H2O
SMILES string
NCCCCNCCCN
UV absorption
λ: 260 nm Amax: 0.1, λ: 280 nm Amax: 0.05
suitability
suitable for molecular biology
application(s)
advanced drug delivery
genomic analysis
InChI
1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2
InChI key
ATHGHQPFGPMSJY-UHFFFAOYSA-N
Gene Information
rat ... Ppm1a(24666)
General description
スペルミジンはポリアミンであり、3価の有機カチオンです。スペルミジンは、自然に生じるポリアミン化合物で、さまざまな生化学的特性を有します。遺伝子発現において重要な役割を果たし、DNA複製、転写、および翻訳プロセスに関与します。スペルミジンは、細胞成長、増殖、および分化に不可欠であり、細胞周期の進行と組織発達に影響を及ぼします。抗酸化活性を有し、フリーラジカルを除去することで酸化的ストレスから細胞を保護します。また、スペルミジンはオートファジーを刺激して、細胞恒常性のために、損傷した細胞小器官およびタンパク質の凝集体の転換を促進します。その神経保護作用により、ニューロンの生存、シナプスの可塑性、および神経変性疾患の予防をサポートします。さらに、スペルミジンは細胞シグナル伝達経路を調節して、炎症反応と免疫反応に関与します。
Application
スペルミジンは、次の用途で使用されています。
- 染色体単離バッファーの成分として。
- 転写バッファーの成分として。
- ホモジナイズバッファーの成分として。
Biochem/physiol Actions
神経型一酸化窒素合成酵素（nNOS）を阻害します。DNAに結合し沈殿させ、DNA結合タンパク質の精製に使用できます。T4ポリヌクレオチドキナーゼ活性を刺激します。
神経型一酸化窒素合成酵素（nNOS）を阻害します。DNAに結合し沈殿させます。T4ポリヌクレオチドキナーゼ活性を刺激します。
スペルミジンは、細胞の増殖や分化、DNAの安定性やアポトーシスなどのさまざまな細胞活動を調節します。
Features and Benefits
- 汎用性が高く、さまざまな実験用途と研究用途に適用可能
- 分子生物学および生化学の研究に適している
- DNase、RNase、NICKase、およびプロテアーゼフリー
- 重金属汚染物質のレベルが低いことが試験により確認済みで、さまざまな用途への適合性が保証されている
- 陰イオンと陽イオンの量がわずかであることが試験済み
Analysis Note
液体または固体
Disclaimer
滅菌が必要な場合は、オ-トクレ-ブではなく、濾過滅菌を行ってください。スペルミジンは経時的に脱アミノ化するので、溶液は凍結保存してください。溶液は用時調製してください。
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当該品目 | |||
|---|---|---|---|
| description BioUltra, Molecular Biology, ≥99.5% (GC) | description analytical standard | description ≥97% | description ≥99.0% (AT) |
| assay ≥99.5% (GC) | assay ≥99.0% (GC), ≥97.5% (NT) | assay ≥97% | assay ≥99.0% (AT) |
| technique(s) ChIP: suitable, buffer exchange: suitable | technique(s) gas chromatography (GC): suitable, HPLC: suitable | technique(s) - | technique(s) - |
| grade Molecular Biology | grade analytical standard | grade - | grade - |
| suitability suitable for molecular biology | suitability - | suitability - | suitability - |
| solubility H2O: 1 M at 20 °C, clear, colorless | solubility - | solubility water: 50 mg/mL, clear, colorless | solubility H2O: 0.1 g/mL, clear, colorless |
| storage condition (Tightly closed. Keep locked up or in an area accessible only to qualified or authorizedpersons.) | storage condition - | storage condition - | storage condition - |
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注意喚起語
Danger
ハザードコード
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
保管分類
8A - Combustible corrosive hazardous materials
引火点 (F)
233.6 °F - closed cup
引火点（°C）
112 °C - closed cup
PPE（個人用保護具）
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
85558-RSAMPLE: + 85558-1G: + 85558-VAR: + 85558-5G: + 85558-BULK: + 85558-25G: + 85558-INTR:
jan
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How should one provide inert gas atmosphere for storage after opening the provided vial? Do aliquots (diluted to 1M) need to be kept under inert gas as well? How long can one store said aliquots without impairng activity?
1 回答-
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How can I determine the shelf life / expiration / retest date of this product?
1 回答-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf役に立ちましたか？
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 回答-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What's the molar concentration of the product? Is it 1M?
1 回答-
This product is considered a pure material rather than a solution. The concentration can be determined by dividing the density by the molecular weight and correcting for units. This gives 6.37M, which would then need to be multiplied by the batch-specific density for the batch in question, as the material is not 100% pure.
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What is the container for item 85558-1G Spermidine and 43816-10mL DL-Dithiothreitol solution?
1 回答-
Both items are from Buchs. Item 85558-1g comes in a narrow neck glass bottle, 5 ml, amber. Item 43816-10mL is a 15 ml plastic tube made of polypropylene (PP) and includes a sterile cap.
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Under which conditions should this UNopened product be stored? What is the shelf life of an UNopened product? Under which conditions should this OPENED product be stored? What is the shelf life of an OPENED product?
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This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. No change in storage temperature is required after opening provided that the item is handled properly and free of contaminants. This product is not assigned an expiration date. While considered stable, this item may be subject to degradation over time and is assigned a periodic retest date to confirm quality. The lot specific retest date is reported on the Certificate of Analysis. See the link below to review a sample of lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/85558#product-documentation役に立ちましたか？
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spermidine 85558. I have received 1 G in liquid. Can you please tell me 1G in what volume? and what solution? Never mentioned anywhere.
1 回答-
The product is a neat or pure compound with a melting point of approximately room temperature. The material may be received as either a liquid or a solid. The density of this product is 0.925 g/mL and the concentration is 6.4M.
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is this a spermidine solution or a neat liquid, and what is the concentration?
1 回答-
Based on a density of 0.925 g/mL and a molecular weight of 145.25, this neat liquid has an effective molarity of 6.4 M.
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