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詳細
Lugol′s solution is a ready-to-use solution that comprises 6% iodine in 4% potassium iodide. It is commonly used as a mordant for Gram staining in bacteriology. It is used for staining bacterial target structures for diagnostic purposes by fixing, staining, counterstaining, and mounting bacteriological specimen materials.
アプリケーション
- Lugol′s solution is generally used as a mordant in Gram staining, and for fixing invertebrate blood cells and protozoa.
- It is suitable for the detection of starch in plants.
- It has been used in a study to assess narrow-band imaging without image magnification for detecting high-grade dysplasia and intramucosal esophageal squamous cell carcinoma.
- It has been used in a study to determine its benefit in aiding the detection, presence, and spread of small squamous cell carcinomas of the esophagus.
生物化学的／生理学的作用
Iodine diffuses rapidly through hydrophilic and hydrophobic domains of tissues and loosely binds to proteins, staining them brown. It reacts irreversibly with unsaturated lipid linkages and with tyrosine and histidine aromatic rings.
特徴および利点
- Ready-to-use solution.
原理
In bacteriology, Gram staining allows rapid differentiation between Gram-positive and Gram-negative bacteria. The mureine structure of the bacterial cell wall is the basis of the color affinity. Bacteria are first stained with the primary stain, crystal violet. Lugol solution is added thereafter as the mordant, forming crystal violet-iodine complexes. During the decolorization, these complexes stay entrapped in the multilayer mureine structure of the cell wall of Gram-positive bacteria which appear blue-violet. Gram-negative bacteria have a single-layered cell wall that does not retain the dye-iodine complexes and are counterstained with safranine solution, appearing pink.
保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
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労働安全衛生法名称等を表示すべき危険物及び有害物
名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
Jan Code
32922-VAR:
32922-1.5L:
32922-250ML:
32922-6X250ML:
32922-BULK:
32922-1L:
32922-INTR:
32922-6X1L:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
この製品を見ている人はこちらもチェック
Histological and Histochemical Methods: Theory and Practice
J.A. Kiernan
Histological and Histochemical Methods: Theory and Practice (2015)
Jason M Cole et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(25), 6335-6340 (2018-06-07)
In the field of X-ray microcomputed tomography (μCT) there is a growing need to reduce acquisition times at high spatial resolution (approximate micrometers) to facilitate in vivo and high-throughput operations. The state of the art represented by synchrotron light sources
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