306-05A

Human Cardiac Fibroblasts: HCF, adult

• 別名: HCF cells
• UNSPSCコード: 41106514
• NACRES: NA.81

特性

• 由来生物: human heart (normal adult)
• 包装: pkg of 500,000 cells
• メーカー／製品名: Cell Applications, Inc
• 成長モード: adherent
• 核型: 2n = 46
• 形態: fibroblast
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cardiovascular diseases
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

詳細

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Cardiac fibroblasts are the most prevalent cell type in the heart, making up 60-70 % of all cells. HCF from Cell Applications, Inc. provide an excellent model system to study many aspects of human heart function and pathophysiology.

HCF has been utilized in a number of research publications, for example to:

• Determine that electrical coupling between cardiomyocytes and fibroblasts is mediated by large-conductance Ca²⁺-activated K⁺ channels that can be stimulated by estrogen receptor agonists (Wang, 2006, 2007); and show that antimitogenic effects of estradiol on HCF growth are mediated by cytochromes 1A1/1B1-and catechol-O-methyltransferase-derived metabolites (Dubey, 2005)
• Show that in response to mechanical stretch, cardiac fibroblasts release TGF-b which causes trombomodulin downregulation, increasing the risk of thromboembolic events (Kapur, 2007) and also induces cardiac fibroblast differentiation into myofibroblasts via increased Smad2 and ERK1/2 phosphorylation, that could be stimulated by endothelin-1 and inhibited by Ac-SDKP (Peng, 2010)
• Demonstrate that activation of G protein-coupled receptor kinase-2 (GRK2) prevents normal regulation of collagen synthesis in cardiac fibroblasts mimicking heart failure phenotype (D’Souza, 2011); identify FGF2 signaling pathway as potential target for modulating apoptosis in cardiac pathology (Ma, 2011) and investigate the roles of scleraxis (Bagchi, 2012) and AMPKa1 (Noppe, 2014) in scar formation following myocardial infarction
• Show that the KATP channel opener KMUP-3 preserved cardiac function after myocardial infarction by enhancing the expression of NO synthase and restoring MMP-9/TIMP-1 balance (Liu, 2011)

HCF were also shown to express delayed rectifier IK, Ito, Ca²⁺-activated K⁺ current (BKCa), inward-rectifier (Kir-type), and swelling-induced Cl- current (ICl.vol) channels (Yue, 2013).

細胞株の由来

Heart

アプリケーション

human heart function, neointimal formation, vascular remodeling, myocardial repair, extracellular maintenance, signaling, toxicology, gene expression, structural support for cardiomycytes, synthesis of extracellular matrix, growth factors and cytokines

構成

Basal Medium containing 10% FBS & 10% DMSO

調製ノート

• 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 8 doublings

継代と培養方法

Please refer to the HCF Culture Protocol.

免責事項

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable