PPB015

Tris-Glycine Buffer

pH 8.3, pHast Pack^™, powder

• 別名: 1X TG buffer, TG, TG Buffer, Tris Glycine running buffer, Tris Glycine transfer buffer, Tris glycine
• UNSPSCコード: 41105319
• NACRES: NA.25

特性

• 製品種目: pHast Pack^™
• 品質水準: 100
• 形状: powder
• 保管条件: (Tightly closed. Dry)
• 不純物: ≤5 ppm Heavy Metals (lead)
• 色: white to off-white
• pH: 8.3
• 溶解性: soluble, clear, colorless to faintly yellow
• 微量陽イオン: Fe: <10 ppm
• その他の活性: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• 保管温度: room temp

詳細

詳細

Tris-Glycine buffer is primarily used as a running buffer and to prepare transfer buffer in western blot analysis with the addition of methanol. This buffer can separate proteins by their size. It has a pH of 8.3.

アプリケーション

Suitable for use as:

• Transfer buffer component in western blot
• Running buffer in native gel electrophoresis for protein separation
• Running buffer in gradient polyacrylamide gel electrophoresis (PAGE)

特徴および利点

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Saves time and minimizes the effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

包装

Foil pouches

再構成

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 25 mM Tris, 192 mM Glycine, pH 8.3 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

保管および安定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

その他情報

For additional information on our range of Biochemicals, please complete this form.

法的情報

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1