PPB005

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween^® 20, pHast Pack^™

• 別名: PBS + 0.05% TWEEN^® 20, PBS with 0.05% Tween 20, PBS-T, PBST
• UNSPSCコード: 12161700
• NACRES: NA.21

特性

• 製品名: 0.05％TWEEN^®20を含むリン酸緩衝生理食塩水（pH7.4）, pHast Pack^™, powder
• 製品種目: pHast Pack^™
• フォーム: powder
• 保管条件: (Tightly closed. Dry)
• 不純物: ≤5 ppm Heavy Metals (lead)
• 色: white
• pH: 7.2—7.6 (25 °C)
• 溶解性: soluble, clear to hazy, colorless
• 微量陽イオン: Fe: <10 ppm
• その他の活性: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• 保管温度: room temp

詳細

詳細

Phosphate-buffered saline with 0.05% Tween^® 20 or PBST contains PBS buffer with a low-concentration of Tween detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.

アプリケーション

PBST is used in research in a variety of assays including DNA or RNA hybridization assays and cell culture. It is commonly used for immunoassays like Western blot, ELISA, Dot blot, Immunohistochemistry (IHC), immunopercipitation (IP), and immunofluorecence. It has been used for incubation and wash buffer for peptide microarrays for performing High-density peptide array (HDPA) to analyze antibody responses against SARS-CoV-2[1].

特徴および利点

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

包装

Foil pouches

再構成

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

保管および安定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

その他情報

For additional information on our range of Biochemicals, please complete this form.

法的情報

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable