67438

C. perfringens Millichrome^™ plus Agar Base

selective for bacteria: Clostridium perfringens, suitable for microbiology

• 別名: C. perfringens Chromogenic Agar Base, C. perfringens Differential Agar Base, C. perfringens Selective Agar Base
• UNSPSCコード: 41161501
• NACRES: NA.74

特性

• 認証: EP; JP
• 品質水準: 200
• 滅菌適合性: autoclavable compatible
• フォーム: dry powder
• シェルフライフ: limited shelf life, expiry date on the label
• 組成: Agar, 15.0 g/L ; Chromogenic and Selective mix, 1.4 g/L ; Peptones and yeast; extract, 25.0 g/L ; Salts, 6.0 g/L
• メーカー／製品名: MilliChrome^™
• テクニック: microbiological culture: suitable
• 最終pH: 7.6±0.2 (25 °C)
• 溶解性: deionized water: 50.9 g/L
• アプリケーション: food and beverages; pharmaceutical; water monitoring; microbiology
• 輸送温度: ambient
• 保管温度: 15-25°C
• 適合性: selective for bacteria: Clostridium perfringens

詳細

詳細

C. perfringens Millichrome^™ plus Agar Base is a selective chromogenic medium used for the detection and enumeration of Clostridium perfringens. The medium contains peptones and yeast extract that provide nitrogenous nutrients for growth, sodium chloride for osmotic balance, chromogenic substrates for color differentiation, and selective agents to inhibit the growth of other organisms. The medium also contains some special growth factors for the growth of C. perfringens, and agar as the solidifying agent.

アプリケーション

C. perfringens Millichrome^™ plus Agar Base is suitable for the detection and enumeration of Clostridium perfringens.

調製ノート

Step 1 (Preparation of the base C. perfringens Millichrome^™ plus Agar Base)

• Disperse slowly 50.9 g of powder base in 1 L of purified water.
• Stir until agar is well thickened.
• Heat and bring to boiling (100 °C) while swirling or stirring regularly.
• AUTOCLAVE at 121 °C during 15 min.
• Mix when removing it from the autoclave.
• Cool in a water bath to 45/50 °C, swirling or stirring gently.

Step 2 (Preparation of the Supplement 1)

• In a transparent vessel, add 2 g of C. perfringens Millichrome^™ plus Agar Base supplement 1 in 20 mL of purified water.
• Swirl well until complete dissolution.
• Filter to sterilize at 0.45 μm.

Step 3 (Preparation of the Supplement 2)

• Add 0.12 g of C. perfringens Millichrome^™ plus Agar Base supplement 2 in 1 mL of purified water.
• Filter to sterilize at 0.45 μm

Step 4 (Mixing of the prepared base and the supplements 1 and 2)

• Add 20 mL/L of the supplement 1 solution to the melted base at 45/50 °C.
• Add 1 mL/L of the supplement 2 solution to the melted base at 45/50 °C.
• Swirl or stir gently to homogenize.
• Pour into sterile Petri dishes
• Let it solidify and dry.

保管および安定性

• Store prepared media below 8°C, protected from direct light (max. 1 month).
• Store dehydrated powder, in a dry place, in tightly sealed containers at 2-25°C.

アナリシスノート

The medium is specific for C. perfringens and can differentiate it from other organisms based on the orange color of the colonies.

法的情報

MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3