おすすめの製品
品質水準
使用法
sufficient for 100 reactions
sufficient for 1000 reactions
sufficient for 500 reactions
sufficient for 5000 reactions
シェルフライフ
≤12 mo.
特徴
dNTPs included
hotstart
包装
kit of 1 mL (100 x 20 μL rxn; KK4603)
kit of 10 mL (1000 x 20 μL rxn; KK4605)
kit of 5 mL (500 x 20 μL rxn; KK4604)
kit of 50 mL (5000 x 20 μL rxn; KK4617)
メーカー／製品名
Roche
濃度
2 ×
テクニック
qPCR: suitable
入力
purified DNA
適合性
for use with Applied Biosystems® 5700
for use with Applied Biosystems® 7000
for use with Applied Biosystems® 7300
for use with Applied Biosystems® 7700
for use with Applied Biosystems® 7900HT
for use with StepOne™
for use with StepOnePlus™
検出方法
SYBR® Green
保管温度
−20°C
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詳細
KAPA SYBR® FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition.
The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.
The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.
アプリケーション
KAPA SYBR® FAST qPCR kit can be used for:
- Gene expression
- Gene knockdown validation
- Microarray validation
- ChIP analysis
- Low copy detection
- Absolute quantification of NGS libraries
- Real-time PCR
特徴および利点
Quantitate changes in gene expression more accurately
Detect low copy and difficult targets consistently
Complete real-time PCR runs in just 40 minutes
Quick Notes:
- High reaction efficiency between 95 – 105% improves accuracy and reproducibility.
- Unbiased efficiency across a wide range of GC contents and amplicon lengths.
Detect low copy and difficult targets consistently
- Improved processivity results in earlier Cq scores.
- Higher fluorescence detection across varying AT- and GC-rich targets.
- Novel enzyme is resistant to SYBR® inhibition.
Complete real-time PCR runs in just 40 minutes
- 55% shorter run times with fast cycling protocol.
- Maintain high performance when switching from standard to fast protocols.
Quick Notes:
- This kit contains an engineered enzyme optimized for qPCR using SYBR Green I dye chemistry.
- The 2X master mix contains a proprietary buffer. Together with the novel enzyme, this improves amplification efficiency of both GC- and AT-rich targets.
- 20 sec initial denaturation at 95°C is sufficient for enzyme activation. When working with complex templates, an initial denaturation of 3 min is recommended.
- For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition at 72°C.
- Do not exceed 25 μL reaction volumes.
品質
All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo and endonuclease activity, and meet strict requirements with respect to DNA contamination.
調製ノート
Always ensure that components have been fully thawed and thoroughly mixed before use. KAPA SYBR® FAST qPCR Master Mix (2X) may not freeze solidly, even when stored at -20°C.
The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix (2X) ABI PRISM® and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.
KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week and may be stored at this temperature for short-term use, provided that they do not become contaminated with microbes and/or nucleases.
The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix (2X) ABI PRISM® and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.
KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week and may be stored at this temperature for short-term use, provided that they do not become contaminated with microbes and/or nucleases.
その他情報
For Research Use Only. Not for use in diagnostic procedures.
法的情報
ABI PRISM is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SYBR is a registered trademark of Life Technologies
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
キットの構成要素のみ
製品番号
詳細
- KAPA SYBR® FAST DNA Polymerase
- reaction buffer
- dNTPs
- SYBR® Green I dye
- MgCl2 2.5 mM
シグナルワード
Warning
危険有害性情報
危険有害性の分類
STOT SE 2
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
does not flash
引火点(℃)
does not flash
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
MicroRNA-138 suppresses ovarian cancer cell invasion and metastasis by targeting SOX4 and HIF-1a
Yu-Ming Yeh
International Journal of Cancer. Journal International Du Cancer, 133(4), 867-878 (2013)
CD1d induction in solid tumor cells by histone deacetylase inhibitors through inhibition of HDAC1/2 and activation of Sp1.
Yang Pei-Ming, et al.
Epigenetics, 7(4), 390-399 (2012)
SMN is required for the maintenance of embryonic stem cells and neuronal differentiation in mice.
Chang W F, et al.
Brain Structure &Amp; Function, 220(3), 1539-1553 (2015)
Establishment of alternative culture method for spermatogonial stem cells using knockout serum replacement.
Aoshima K, et al.
PLoS ONE, 8(10), e77715-e77715 (2013)
Pei-Ming Yang et al.
Epigenetics, 7(4), 390-399 (2012-03-16)
CD1d is a MHC class-like molecule that presents glycolipids to natural killer T (NKT) cells, then regulates innate and adaptive immunity. The regulation of CD1d gene expression in solid tumors is still largely unknown. Gene expression can be epigenetically regulated
資料
An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
指向性進化の概要と最適化された、またはまったく新しい機能を有するタンパク質の生成方法。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）