SCC119
ImKC Immortalized Mouse Kupffer Cell Line
The ImKC Murine Immortalized Kupffer Cell Line can be used to develop in vitro model cell systems to advance the study of liver injury, function, and disease.
別名:
ImKC Cell Line
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About This Item
UNSPSCコード:
41106514
eCl@ss:
32011203
NACRES:
NA.81
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由来生物
mouse
テクニック
cell based assay: suitable
cell culture | mammalian: suitable
輸送温度
ambient
詳細
ImKC is a cytokine-producing immortalized Kupffer cell line that was established from transgenic mice expressing the thermolabile mutant tsA58 of the Simian virus 40 large T antigen under the control of the H-2kb promoter. Primary KC were obtained using a three step procedure: liver perfusion, centrifugal elutriation, and sorting for F4/80+ cells. ImKC were identified within the small-intermediate population of KC that maintained stable expression of F4/80, and the surface antigens CD11b, CD14 and TLR4.
Kupffer cells (KC), also known as stellate macrophages, are liver macrophages that reside in the lumen of the liver sinusoids. Kupffer cells play a critical role in maintaining liver functions including the removal of pathogens & foreign materials, antigen presentation and modulating innate immune responses. In vitro studies of Kupffer cells have been hindered by the difficulty in isolating sufficient number of primary cells and the limited lifespan of the cells in culture.
細胞株の説明
Macrophage Cells
アプリケーション
Research Category
炎症及び免疫
代謝
炎症及び免疫
代謝
Research Sub Category
肝生理学
肝生理学
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
品質
• Each vial contains ≥ 1X106 viable cells.
• Cells tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma contamination
• Cells tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma contamination
保管および安定性
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SCC119:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Eui Jin Kim et al.
Pharmaceutics, 13(3) (2021-04-04)
Inflammation is a defense mechanism that protects the body from infections. However, chronic inflammation causes damage to body tissues. Thus, controlling inflammation and investigating anti-inflammatory mechanisms are keys to preventing and treating inflammatory diseases, such as sepsis and rheumatoid arthritis.
Julia Rudolph et al.
Scientific reports, 11(1), 15702-15702 (2021-08-05)
Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial
Hepatocyte SREBP signaling mediates clock communication within the liver.
Guan, et al.
The Journal of Clinical Investigation, 133 (2023)
Hsin-Tzu Liu et al.
Antioxidants (Basel, Switzerland), 11(5) (2022-05-29)
Inflammation plays an important role in the pathophysiology of depression. This study aims to elucidate the antidepressant effect of baicalein, an anti-inflammatory component of a traditional Chinese herbal medicine (Scutellaria baicalensis), on lipopolysaccharide (LPS)-induced depression-like behavior in mice, and to
Zheng-Yu Wang et al.
Cytokine, 70(2), 165-172 (2014-08-21)
Kupffer cells (KC) play a critical role in both liver physiology and the pathogenesis of various liver diseases. Isolated primary KC have a limited lifespan in culture, and due to the relatively low number obtained, limit their study in vitro.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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