おすすめの製品
product name
CR4 Human Colorectal Cancer Cell Line, CR4 is a unique, cancer stem cell (CSC) enriched, human colorectal cancer cell line useful for CSC-targeted drug development and research focused on cancer cell and CSC biology.
由来生物
human
品質水準
詳細
CR4 is a novel CIC-enriched, highly tumorigenic, clonogenic and 3D spheroid-forming colon cancer cell line derived from the liver metastasis of a male patient with stage 4 colon cancer (1). Primary tumor cells were established on type I collagen coated flasks in serum-free stem cell media (1). Holoclones, small stem-like cells that are densely packed, were selectively subcloned from the heterogeneous culture and propagated. Cells were then passaged as 3D spheroids and in NOD/SCID mice xenografts leading to the eventual establishment of the CR4 human colorectal cancer cell line.
Majority of CR4 cells express high but variable levels of common cancer stem cell markers including CD44, CD166, EpCAM, and Lgr51. CR4 cell line has the G12V mutation on KRAS exon 2 (Botchkina GI, communications).
Reference:
1. Rowehl RA, Burke S, Bialkowska AB, Pettet DW III, Rowehl L, Li E, Antoniou E, Zhang Y, Bergamaschi R, Shroyer KR, Ojima I., Botchkina GI (2014) PLOS 9(6): e99091.
Majority of CR4 cells express high but variable levels of common cancer stem cell markers including CD44, CD166, EpCAM, and Lgr51. CR4 cell line has the G12V mutation on KRAS exon 2 (Botchkina GI, communications).
Reference:
1. Rowehl RA, Burke S, Bialkowska AB, Pettet DW III, Rowehl L, Li E, Antoniou E, Zhang Y, Bergamaschi R, Shroyer KR, Ojima I., Botchkina GI (2014) PLOS 9(6): e99091.
Colorectal cancer (CRC) is the 2nd highest cause of cancer death in the United States. Increasing evidence suggests that a small population of cancer-initiating cells (CICs) or cancer stem cells (CSCs) are responsible for tumorigenesis and the exceptional drug resistance to anti-cancer therapies.
品質
• Each vial contains ≥1X10⁶ viable cells.
• Cells are tested negative for HPV-16, HPV-18, Hepatitis A, B, C, Herpesvirus type 7, 8 and HIV-1 & 2 viruses by PCR
• Cells are positive for herpesvirus type 6 by PCR analysis.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
• Cells are tested negative for HPV-16, HPV-18, Hepatitis A, B, C, Herpesvirus type 7, 8 and HIV-1 & 2 viruses by PCR
• Cells are positive for herpesvirus type 6 by PCR analysis.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
その他情報
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the "Academic Use Agreement" as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
免責事項
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SCC107:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）