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Merck

SCC009

Sigma-Aldrich

ReNcell CX Human Neural Progenitor Cell Line & Culture Media Kit

ReNcell CX Kit contains human neural progenitor cells, optimized maintenance media & freezing media for culture of human neural stem cells.

別名:

Human NSC Line, Human Neural Stem Cell Line, Human Neural Stem Cells

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About This Item

UNSPSCコード:
41106509
eCl@ss:
32011203
NACRES:
NA.75

由来生物

human

品質水準

メーカー/製品名

Chemicon®
ReNcell

テクニック

cell culture | stem cell: suitable

入力

sample type neural stem cell(s)

輸送温度

liquid nitrogen

関連するカテゴリー

詳細

ReNcell CX Kit contains human neural progenitor cells, optimized maintenance media and freezing media for culture of human neural stem cells. ReNcell CX Immortalized Cells (SCC007): ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell CX was derived from the cortical region of human fetal brain. Immortalized by retroviral transduction with the c-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell CX retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). ReNcell CX was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. ReNcell CX may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ReNcell CX cells have been obtained in a legal and ethical manner, compliant with current local informed consent procedures. ReNcell NSC Maintenance Medium (SCM005): ReNcell Neural Stem Cell (NSC) Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell immortalized VM and CX neural stem cells. Withdrawal of the growth factors from ReNcell NSC Maintenance Medium will result in the spontaneous differentiation of ReNcell immortalized neural progenitor cells. ReNcell NSC Freezing Medium (SCM007): ReNcell NSC Freezing Medium is qualified for use with ReNcell immortalized human neural progenitor cell lines, CX (CHEMICON Catalog No. SCC007) and VM (CHEMICON Catalog No. SCC008) cultured in serum-free conditions with ReNcell NSC Maintenance Medium (CHEMICON Catalog No. SCM005). The optimized formulation allows for consistent cryopreservation and high viability upon thawing and plating.

細胞株の説明

Neural Stem Cells

アプリケーション

Research Category
幹細胞研究

構成

ReNcell Neural Stem Cell Freezing Medium (Cat.# SCM007)

ReNcell NSC Maintenance Media (Cat.# SCM005)

ReNcell CX Immortalized Cell Line (Cat.# SCC007)

品質

Each lot of ReNcell CX cells has been validated for high level of expression of Nestin and Sox 2 and for their self-renewal and multi-lineage differentiation capacities (please refer to datasheet figures). Cells also display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma. Maternal blood has been screened for HIV, HTLV, Hepatitis B and C.

その他情報

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the "Academic Use Agreement" as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

保管分類コード

10 - Combustible liquids


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

SCC009:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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Panpan Dong et al.
Experimental and therapeutic medicine, 23(2), 164-164 (2022-01-25)
Bisphenol A (BPA) is a common industrial chemical widely used to produce various plastics and is known to impair neural stem cells (NSCs). However, the effects of low-dose BPA exposure on the stemness maintenance and differentiation fate of NSCs remain
Differential development of neuronal physiological responsiveness in two human neural stem cell lines.
Donato, R; Miljan, EA; Hines, SJ; Aouabdi, S; Pollock, K; Patel, S; Edwards, FA; Sinden, JD
BMC Neuroscience null
Seong Won Lee et al.
Developmental cell, 46(1), 73-84 (2018-07-06)
The ability to convert human somatic cells efficiently to neurons facilitates the utility of patient-derived neurons for studying neurological disorders. As such, ectopic expression of neuronal microRNAs (miRNAs), miR-9/9∗ and miR-124 (miR-9/9∗-124) in adult human fibroblasts has been found to

資料

Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling

プロトコル

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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