おすすめの製品
由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
形状
liquid
含みます
≤0.1% Sodium azide as preservative
化学種の反応性
Drosophila, hamster, rat, human, monkey, mouse
メーカー／製品名
Calbiochem®
保管条件
OK to freeze
avoid repeated freeze/thaw cycles
アイソタイプ
IgG
輸送温度
wet ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
詳細
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~35 kDa CHIP protein.
Recognizes the ~35 kDa CHIP protein. May also detect bands at ~43, ~51, and ~70 kDa corresponding to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively.
This Anti-CHIP Rabbit pAb is validated for use in Immunoblotting for the detection of CHIP.
免疫原
a recombinant protein consisting of full-length human CHIP fused to a His•Tag sequence
アプリケーション
Immunoblotting (1:200-1:1000)
Immunofluorescence (1:250)
Immunoprecipitation (1:20)
Immunofluorescence (1:250)
Immunoprecipitation (1:20)
警告
Toxicity: Standard Handling (A)
物理的形状
Undiluted serum.
再構成
Following initial thaw, aliquot and freeze (-20°C).
アナリシスノート
Positive Control
COS-7 cells
COS-7 cells
その他情報
May also detect bands at 43, 51, and 70 kDa, wich correspond to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively. Antibody should be titrated for optimal results in individual systems.
Xu, W., et al. 2002. Proc. Natl. Acad. Sci. USA99, 12847.
Demand, J., et al. 2001. Curr. Biol.11, 1569.
Connell, P., et al. 2001. Nat. Cell Biol.3, 93.
Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
Meacham, G.C., et al. 2001. Nat. Cell. Biol.3, 100.
Ballinger, C.A., et al. 1999. Mol. Cell. Biol.19, 4535.
Demand, J., et al. 2001. Curr. Biol.11, 1569.
Connell, P., et al. 2001. Nat. Cell Biol.3, 93.
Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
Meacham, G.C., et al. 2001. Nat. Cell. Biol.3, 100.
Ballinger, C.A., et al. 1999. Mol. Cell. Biol.19, 4535.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
PC711-100UL:
PC711-UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Britney N Lizama et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(31), 6825-6840 (2018-06-24)
The C terminus of HSC70-interacting protein (CHIP, STUB1) is a ubiquitously expressed cytosolic E3-ubiquitin ligase. CHIP-deficient mice exhibit cardiovascular stress and motor dysfunction before premature death. This phenotype is more consistent with animal models in which master regulators of autophagy
Nadja Kettern et al.
PloS one, 6(1), e16398-e16398 (2011-02-02)
The maturation of mouse macrophages and dendritic cells involves the transient deposition of ubiquitylated proteins in the form of dendritic cell aggresome-like induced structures (DALIS). Transient DALIS formation was used here as a paradigm to study how mammalian cells influence
Jeannette N Stankowski et al.
Antioxidants & redox signaling, 14(10), 1787-1801 (2010-08-04)
The decision to remove or refold oxidized, denatured, or misfolded proteins by heat shock protein 70 and its binding partners is critical to determine cell fate under pathophysiological conditions. Overexpression of the ubiquitin ligase C-terminus of HSC70 interacting protein (CHIP)
Xinjia Wang et al.
Molecular endocrinology (Baltimore, Md.), 19(6), 1474-1482 (2005-03-12)
The ubiquitin/proteasome-dependent protein degradation pathway (UPP) is responsible for the accelerated down-regulation of glucocorticoid receptor (GR) levels in cells subjected to chronic glucocorticoid exposure. Whereas hormone-dependent down-regulation of GR operates in most cells, the receptor is not down-regulated after long-term
Rudolf A Kley et al.
Brain : a journal of neurology, 135(Pt 9), 2642-2660 (2012-09-11)
Mutations in FLNC cause two distinct types of myopathy. Disease associated with mutations in filamin C rod domain leading to expression of a toxic protein presents with progressive proximal muscle weakness and shows focal destructive lesions of polymorphous aggregates containing
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