MABT1510

Anti-MBP (Citrulline R25) Antibody, clone 1B8

clone 1B8, from mouse

• 別名: Myelin basic protein, Myelin A1 protein, Myelin membrane encephalitogenic protein, Citrullinated MBP
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 1B8, monoclonal
• 化学種の反応性: human, mouse
• 包装: antibody small pack of 25 μg
• テクニック: ELISA: suitable; electron microscopy: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NP_001020252
• UniProtアクセッション番号: P02686
• ターゲットの翻訳後修飾: citrullination (Arg25)

詳細

詳細

Myelin basic protein (UniProt: P02686; also known as MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein) is encoded by the MBP gene (Gene ID: 4155) in human. MBP is a homodimeric protein that is found in both the central and the peripheral nervous system. It is one of the most abundant protein component of the myelin membrane in the CNS and plays a role in both the formation and stabilization of this myelin membrane. It is responsible for adhesion of the cytosolic surfaces of multilayered compact myelin. MBP can interact with many polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and with negatively charged lipids. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. MBP expression begins abruptly in 14-16-week-old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. MBP citrullination by peptidylarginine deiminase (PAD) leads to incomplete protein-lipid bilayer interactions and increases its vulnerability to proteolytic enzymes, resulting in disorganization of the myelin sheath in the central nervous system. Citrullinated MBP (citMBP) has been suggested to be a hallmark of demyelination. MBP citrullination has frequently been demonstrated in various CNS disorders. citMBP at R25, R122, and R130 is reported in the brains of subjects with Creutzfeldt-Jakob disease (CJD). Clone 1B8 specifically recognizes citMBP at R25. (Ref.: Jang, B., et al. (2017). Mol. Neurobiol. 55(4); 3172-3184; Boggs, JM (2006). Cell. Mol. Life Sci. 63(17); 1945-1961).

特異性

Clone 1B8 is a mouse monoclonal antibody that detects human and murine myelin basic protein citrullinated on residue 25. It targets an epitope with in 18 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 18 amino acids from isoform 5 of human Myelin Basic Protein with citrullination on residue 25.

アプリケーション

Research Category
細胞骨格

Anti-MBP (Citrulline R25), clone 1B8, Cat. No. MABT1510, is a mouse monoclonal antibody that detects is Myelin basic protein citrullinated on residue 25 and has been tested for use in ELISA, Electron Microscopy, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected MBP (Citrulline R25) in Immunohistochemistry applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

Electron Microscopy Analysis: A representative lot detected MBP (Citrulline R25) in Electron Microscopy applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MBP (Citrulline R25) in Myelin basic protein (MBP) treated with human PAD2 (Courtesy of Byungki Jang, Ph.D./Eun-Kyoung Choi, Ph.D., Ilsong Institute of Life Science, Hallym University, South Korea).

Immunofluorescence Analysis: A representative lot detected MBP (Citrulline R25) in Immunofluorescence applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

Western Blotting Analysis: A representative lot detected MBP (Citrulline R25) in Western Blotting applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

ELISA Analysis: A representative lot detected MBP (Citrulline R25) in ELISA applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

品質

Evaluated by Western Blotting in Myelin basic protein (MBP) either treated with human PAD-2.

Western Blotting Analysis: 1 µg/mL of this antibody detected Myelin basic protein (MBP) treated with human human Peptidyl Arginine Deiminase 2 (PAD-2) (200 ng for 4 hours at 37°C) with R25 citrullination.

ターゲットの説明

~19 kDa observed; 18.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.