MABS2050

Anti-Factor XII Antibody, clone 9A2

clone 9A2, from mouse

• 別名: Coagulation factor XII, EC: 3.4.21.38, Hageman factor, HAF
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 9A2, monoclonal
• 化学種の反応性: human, baboon
• 包装: antibody small pack of 25 μg
• テクニック: ELISA: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_000496
• UniProtアクセッション番号: P00748
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... F12(2161)

詳細

詳細

Coagulation factor XII (UniProt: P00748; also known as EC: 3.4.21.38, Hageman factor, HAF) is encoded by the F12 gene (Gene ID: 2161) in human. Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates factor XI to XIa and factor VII to VIIa by selective cleavage of Arginine-Isoleucine bond. Factor XII itself can be activated to factor XIIa either by exposure to negatively charged surfaces or via proteolytic cleavage. It is synthesized in the liver with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. Coagulation factor XII circulates in blood as a single chain zymogen, which is converted to a two-chain serine protease with a heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain, and a proline-rich domain. The light chain contains only a catalytic domain. Mutations in F12 gene can lead to Factor XII deficiency that is generally an asymptomatic anomaly of in vitro blood coagulation. However, some mutations may cause hereditary angioedema 3, which is characterized by episodic local swelling involving subcutaneous or submucous tissue of the upper respiratory and gastrointestinal tracts, face, extremities, and genitalia.

特異性

Clone 9A2 is a mouse monoclonal antibody that specifically detects Coagulation factor XII.

免疫原

A mixture of purified human Factor XII and Factor XIIa.

アプリケーション

Research Category
細胞シグナル伝達

Anti-Factor XII, clone 9A2, Cat. No. MABS2050, is a mouse monoclonal antibody that detects Coagulation factor XII and has been tested for use in ELISA, Inhibition and Function assay, and Western Blotting.

ELISA Analysis: A representative lot detected Factor XII in ELISA applications (Matafonov, A., et. al. (2014). Blood. 123(11):1739-46).

Western Blotting Analysis: A representative lot detected Factor XII in Western Blotting applications (Matafonov, A., et. al. (2014). Blood. 123(11):1739-46).

Inhibition Analysis: A representative lot inhibited the generation of thrombin in normal plasma following addition of activated partial thromboplastin reagent. (Matafonov, A., et. al. (2014). Blood. 123(11):1739-46).

Function Analysis: A representative lot prolong the activated partial thromboplastin time. (Matafonov, A., et. al. (2014). Blood. 123(11):1739-46).

品質

Evaluated by Western Blotting in human factor XII.

Western Blotting Analysis: 4 µg/mL of this antibody detected Factor XII in human factor XII.

ターゲットの説明

~75 kDa observed; 67.79 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in PBS, pH 7.4, without preservatives.

保管および安定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.