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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
RAS 10, monoclonal
化学種の反応性
human
テクニック
immunocytochemistry: suitable
western blot: suitable
アイソタイプ
IgG2aκ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... KRAS(3845)
詳細
The human ras gene family consists of three identified members H-, K- and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons. Human H- and K-ras are the homologues of v-H- and v-K-ras, sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging.
免疫原
Recombinant protein corresponding to human Pan-Ras.
アプリケーション
Research Category
細胞シグナル伝達
細胞シグナル伝達
Research Sub Category
成長因子及び受容体
成長因子及び受容体
Detect Pan-Ras using this Anti-Pan-Ras Antibody, clone RAS 10 validated for use in Western Blotting, ICC.
Western Blot Analysis: A representative lot of this antibody detected Pan-Ras in SW-480 cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Pan-Ras in A431 cells.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Pan-Ras in IP (Hamer, P.J., et al. (1990). Hybridoma. 9(6):573-587.).
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Pan-Ras in A431 cells.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Pan-Ras in IP (Hamer, P.J., et al. (1990). Hybridoma. 9(6):573-587.).
品質
Evaluated by Western Blot in MCF7 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Pan-Ras in 10 µg of MCF7 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Pan-Ras in 10 µg of MCF7 cell lysate.
ターゲットの説明
~21 kDa observed
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
アナリシスノート
Control
MCF7 cell lysate
MCF7 cell lysate
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABS195:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Won-Ji Ryu et al.
Experimental & molecular medicine, 52(5), 832-842 (2020-05-28)
Triple-negative breast cancer (TNBC) is a severe and heterogeneous disease that lacks an approved targeted therapy and has a poor clinical outcome to chemotherapy. Although the RAS-ERK signaling axis is rarely mutated in TNBC, ~50% of TNBCs show an increased
Michele Caraglia et al.
International journal of cancer, 121(10), 2317-2330 (2007-07-28)
Interferon alpha (IFNalpha) induces an EGF-Ras-->Raf-1-->Erk dependent survival pathway counteracting apoptosis induced by the cytokine. In this paper we have evaluated the effects of the combination between farnesyl-transferase inhibitor (FTI) R115777 and IFNalpha on the growth inhibition and apoptosis of
F Roßner et al.
Oncogenesis, 5, e187-e187 (2016-01-19)
The transcription factor YBX1 can act as a mediator of signals transmitted via the EGFR-RAS-MAPK axis. YBX1 expression has been associated with tumor progression and prognosis in multiple types of cancer. Immunohistochemical studies have revealed dependency between YBX1 expression and
Stephanie N Brosius et al.
Acta neuropathologica, 127(4), 573-591 (2013-11-16)
Malignant peripheral nerve sheath tumors (MPNSTs) are Schwann cell-derived malignancies that arise from plexiform neurofibromas in patients with mutation of the neurofibromin 1 (NF1) gene. We have shown that the growth factor neuregulin-1 (NRG1) also contributes to human neurofibroma and
Sang-Kyu Lee et al.
EMBO reports, 19(12) (2018-11-11)
RAS proteins play critical roles in various cellular processes, including growth and transformation. RAS proteins are subjected to protein stability regulation via the Wnt/β-catenin pathway, and glycogen synthase kinase 3 beta (GSK3β) is a key player for the phosphorylation-dependent RAS
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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