MABS1697

Anti-WISP-3 Antibody, clone 13C4.1

clone 13C4.1, from mouse

• 別名: WNT1-inducible-signaling pathway protein 3, CCN family member 6, WISP-3
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 13C4.1, monoclonal
• 交差性: mouse, human, rat
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_003871
• UniProtアクセッション番号: O95389
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CCN6(8838)

詳細

詳細

WNT1-inducible-signaling pathway protein 3 (UniProt O95389; also known as CCN family member 6, WISP-3) is encoded by the WISP3 (also known as CCN6, LIBC, PPAC, PPRD, UNQ462/PRO790/PRO956) gene (Gene ID 8838) in human. WISP-3 is a member of the CCN (CTGF, Cyr61, NOV) family connective tissue growth modulators involved in cell growth/differentiation during various phases of development through a complex interplay with BMP/Notch/Wnt signaling pathways. WISP-3 is expressed in cells and tissues of mesenchymal origin and WISP3 gene mutations are linked to an autosomal recessive skeletal disorder known as progressive pseudorheumatoid dysplasia (PPRD), indicating its importance in skeletal growth and the maintenance of cartilage integrity. WISP-3 interacts with BMP and components of the Wnt1 signaling pathway including LRP6 and Fz8, promoting the expression of cartilage-specific proteins collagen-II and aggrecan. Both IGF1 and ROS stimulate WISP-3 expression, WISP-3 in turn binds to and blocks IGF1 secretion, preventing IGF1-induced collagen X induction, ROS accumulation, and alkaline phosphatase activity, all of which are associated with chondrocyte hypertrophy induction. Human WISP-3 is synthesized with a signal peptide sequence (a.a. 1-23), the removal of which yields the mature protein containing several protein-interacting domains, including an IGF-interacting IGFBP domain (a.a. 44-117) with a von Willbrand factor type C repeat, a Thrombospondin (TSP) type-1 domain (a.a. 208-253), and a C-terminal cystine knot-like (CTCK) domain (a.a. 268-342).

特異性

Clone 13C4.1 targets an epitope within the WISP-3 IGFBP domain.

免疫原

GST-tagged recombinant human WISP-3 IGFBP domain fragment.

アプリケーション

Detect WISP-3 using this mouse monoclonal Anti-WISP-3, clone 13C4.1, Cat. No. MABS1697, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50-1,000 dilution of this antibody detected WISP-3 in human liver, kidney, and lung tissue sections.

品質

Evaluated by Western Blotting in rat kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected WISP-3 in 10 µg of rat kidney tissue lysate.

ターゲットの説明

~39 kDa observed. 36.84/36.33 kDa (human/rat mature form), 39.29/41.40 kDa (human isoform 1/2 pro-form), and 38.92 kDa (rat pro-form) calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Format: Purified

その他情報

Concentration: Please refer to lot specific datasheet.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1