MABS1173

Anti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2

clone 10F4.2, from mouse

• 別名: E3 ubiquitin-protein ligase CBL-C, RING finger protein 57, SH3-binding protein CBL-3, SH3-binding protein CBL-C, Signal transduction protein CBL-C
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 10F4.2, monoclonal
• 交差性: human
• テクニック: western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_001124324
• UniProtアクセッション番号: Q9ULV8
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CBLC(23624)

詳細

詳細

E3 ubiquitin-protein ligase CBL-C (UniProt Q9ULV8; also known as RING finger protein 57, SH3-binding protein CBL-3, SH3-binding protein CBL-C, Signal transduction protein CBL-C) is encoded by the CBLC (also known as CBL3, RNF57) gene (Gene ID 23624) in human. CBLC belongs to the CBL family of E3 ubiquitin ligases, members of which also include CBL (a.k.a. c-CBL) and CBL-B. Similar to CBL and CBL-B, CBL-C binds SH3 (Src homology 3) domain-containing proto-oncogenic tyrosine kinases such as EGFR, Lyn, Crk, Src and Ret. CBL-C is recruited to EGFR upon EGF stimulation, leading to CBL-C phosphorylation by EGFR and subsequent downstream MAP kinase signalling. In addition to mediating oncoprotein Ret ubiquitination for proteasomal degradation, CBL-C is shown to form a complex with HIC5 (Hydrogen peroxide-inducible clone 5) and the heat shock protein HSP27 that are necessary for the ubiquitination of NOX4. Unlike the other CBL family members, CBL-C expression appears to be restricted to epithelial tissues. Transgenic expression of CBL-C in mouse mammary gland causes impairment of cell proliferation, suggesting that CBL-C might have a growth-inhibitory role. CBL-C contains an N-terminal Cbl-type phosphotyrosine-binding (Cbl-PTB) domain (a.a. 7-321) that mediates SH3 domain interaction and a C-terminal RET-interacting region (a.a. 351-474) harboring the catalytic ring finger domain (a.a. 351-390) that mediates interaction with E2 ubiquitin-conjugating enzyme. The PTB domain, also known as tyrosine kinase-binding (TKB) domain, is subdivided into a four-helix bundle or 4H domain (a.a. 7-145), a calcium-binding EF hand-like domain (a.a. 146-218), and a SH2-like domain (a.a. 219-321).

特異性

Clone 10F4.2 targets an epitope in the RET-interacting region present in both spliced isoforms of human CBL-C reported by UniProt (Q9ULV8).

免疫原

Epitope: RET-interacting region.

GST-tagged recombinant fragment from the RET-interacting region of human CBL-C.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
ユビキチン及びユビキチン代謝

This Anti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2 is validated for use in Western Blotting for the detection of E3 ubiquitin-protein ligase CBL-C.

品質

Evaluated by Western Blotting in HT-29 human colon cancer cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected CBL-C in 10 µg of HT-29 human colon cancer cell lysate.

ターゲットの説明

~50 kDa observed. 52.46 kDa (isoform 1; Long) and 47.42 kDa (isoform 2; Short) calculated. Uncharacterized band(s) may appear in some lysates.

物理的形状

Protein G purified.

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable