MABN2621

Anti-UNC119 homolog A/mRG4 Antibody, clone A2

• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: A2, monoclonal
• 分子量: calculated mol wt 27.01 kDa; observed mol wt ~28 kDa
• 精製方法: using protein G
• 交差性: mouse
• 包装: antibody small pack of 100 μg
• テクニック: immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• エピトープ配列: Unknown
• タンパク質IDアクセッション番号: NP_035806
• UniProtアクセッション番号: Q9Z2R6
• 輸送温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Unc119(22248)

詳細

詳細

Protein unc-119 homolog A (UniProt: Q9Z2R6; also known as Retinal protein 4, mRG4) is encoded by the Unc119 (also known as Unc119h) gene (Gene ID: 22248) in murine species. UNC119 is an adaptor protein that is involved in the development of the vertebrate nervous system. It is widely expressed among vertebrates and invertebrates and is predominantly localized in photoreceptor synapses in the outer plexiform layer of the retina. It recognizes the acylated N-terminus of the rod photoreceptor transducin -subunit (T ). Apart from its presence in the mammalian retina, it is also detected in leukocytes, T-cells, lung fibroblasts, the adrenal glands, cerebellum, and kidney. UC119 is reported to activate Src-type tyrosine kinases associated with the interleukin-5 receptor and the T-cell receptor by interacting with Src homology domains localized in its N-terminal half. It is also reported to stimulate the induction of -smooth muscle actin and myofibroblast differentiation by TGF-β in human lung fibroblasts. UNC119 can undergo phosphorylation at serine 37, 39, and 41 by casein kinase 2 and these phosphorylations suppresses its interaction with KLHL18 (Kelch Like Family Member 18) and downregulates its KLHL18-mediated degradation. (Ref.: Chaya, T., et al. (2019). EMBO J. 38(23); e101409; Zhang, H., et al. (2011). Nat. Neurosci. 14(7); 874-880; Vepachedu, R., et al. (2007). J. Immunol. 179(1); 682-690).

特異性

Clone A2 is a mouse monoclonal antibody that detects murine UNC119 Homolog A protein (mRG4).

免疫原

His-tagged full-length recombinant mouse UNC119 homolog A (mRRG4).

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in Mouse retina tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UNC119 homolog A (mRG4) in Mouse retina tissue lysate.

Tested Applications

Immunofluorescence Analysis: A representative lot detected UNC119 homolog A/mRG4 in Immunofluorescence applications (Haeseleer, F., et al. (2008). Invest Ophthalmol Vis Sci. 49(6):2366-75).

Western Blotting Analysis: A representative lot detected UNC119 homolog A/mRG4 in Western Blotting applications (Haeseleer, F., et al. (2008). Invest Ophthalmol Vis Sci. 49(6):2366-75).

Immunohistochemistry Applications: A representative lot detected UNC119 homolog A/mRG4 in Immunohistochemistry applications (Haeseleer, F., et al. (2008). Invest Ophthalmol Vis Sci.;49(6):2366-75).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-UNC119 homolog A/mRG4, clone A2, Cat. No. MABN2621, is a mouse monoclonal antibody that detects UNC119 homolog A (mRG4) and is tested for use in Immunohistochemistry, Immunofluorescence, and Western Blotting.

物理的形状

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1