MABE1079

Anti-TAF10/TAFII30 Antibody, clone 6TA-2B11

ascites fluid, clone 6TA-2B11, from mouse

• 別名: Transcription initiation factor TFIID subunit 10, STAF28, TAF(II)30, TAFII-30, TAFII30, Transcription initiation factor TFIID 30 kDa subunit
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: 6TA-2B11, monoclonal
• 化学種の反応性: mouse, human
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_006275
• UniProtアクセッション番号: Q12962
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TAF10(6881)

詳細

詳細

Transcription initiation factor TFIID subunit 10 (UniProt Q12962; also known as STAF28, TAF(II)30, TAFII-30, TAFII30, Transcription initiation factor TFIID 30 kDa subunit) is encoded by the TAF10 (also known as TAF2A, TAF2H, TAFII30) gene (Gene ID 6881) in human.Transcription initiation factor TFIID subunit 10 (UniProt Q12962; also known as STAF28, TAF(II)30, TAFII-30, TAFII30, Transcription initiation factor TFIID 30 kDa subunit) is encoded by the TAF10 (also known as TAF2A, TAF2H, TAFII30) gene (Gene ID 6881) in human. TAF10 (TAFII30) is a subunit of both the general transcription factor (GTF) TFIID and the coactivator SAGA (Spt-Ada-Gcn5-acetyltransferase) histone acetyltransferase (HAT) complexes involved in the initiation of RNA polymerase II (Pol II) transcription in eukaryotes. TFIID complex is composed of the TATA-binding protein (TBP) and thirteen TBP-associated factors (TAFs) that allow its recognition of not only TATA box-containing, but also TATA-less promoters for site-specific assembly of preinitiation complex (PIC). TAF10-knockout studies in mice indicate that TAF10 plays an important role during embryonic development and homeostasis in a tissue-dependent manner. TAF10 ablation is reported to result in severe anemia and lethality at embryonic stage E13.5 due to impaired erythropoiesis. In addition, TAF10 is shown to not only interact with the transcription factor GATA1, but also regulate its transcription as evidenced by its occupancy at GATA1 genomic locus in human fetal erythroid cells.

特異性

Clone 6TA-2B11 (a.k.a. 6TA 2B11) specifically immunostained wild-type, but not Taf10-/-, mouse embryonic blastocysts (Mohan, W.S. Jr, et al. (2003). Mol .Cell. Biol. 23(12):4307-4318).

免疫原

Epitope: Internal (N-terminal half).

Recombinant human TAF10/TAFII30.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
転写因子

Anti-TAF10Antibody, clone 6TA-2B11 is an antibody against TAFII30 for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected TAF10/TAFII30 in A431 and HeLa cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TAF10/TAFII30 in TAFIID complex isolated from HeLa nuclear extract (Courtesy of Dr. Mustapha OULAD-ABDELGHANI, IGBMC, France).
Immunoprecipitation Analysis: A representative lot immunoprecipitated TAF10 together with associated GATA1 from murine erythroleukemia (MEL) cell nuclear extracts (Papadopoulos, P., et al. (2015). Mol. Cell. Biol. 35(12):2103-2118).
Western Blotting Analysis: A representative lot detected TAF10 in E12.5 mouse fetal liver nuclear extracts and murine erythroleukemia (MEL) cell nuclear extracts, as well as in GATA1 immunoprecipitate from MEL cell and fetal liver nuclear extracts (Papadopoulos, P., et al. (2015). Mol. Cell. Biol. 35(12):2103-2118).
Immunocytochemistry Analysis: A representative lot detected TAF10 immunoreactivity in trophoblasts from 3-day cultured wild-type, but not Taf10-/-mouse embryonic blastocysts isolated at 3.5 dpc (days post-coitum) by immunofluorescent staining of 1% paraformaldehyde-fixed, 0.1% Tween 20-permeabilized blastocysts (Mohan, W.S. Jr, et al. (2003). Mol .Cell. Biol. 23(12):4307-4318).

品質

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected TAF10/TAFII30 in 10 µg of A431 cell lysate.

ターゲットの説明

~30 kDa observed. 21.71 kDa calculated.

物理的形状

Unpurified

Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable