MABC1786

Anti-Glypican-2 Antibody, clone CT3

• 別名: GPC2
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: CT3, monoclonal
• 分子量: calculated mol wt 62.83 kDa; observed mol wt ~56 kDa
• 精製方法: using protein G
• 化学種の反応性: human
• 包装: antibody small pack of 100
• テクニック: ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable; inhibition assay: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• エピトープ配列: C-terminal
• タンパク質IDアクセッション番号: NP_689955
• UniProtアクセッション番号: Q8N158
• 保管温度: -10 to -25°C
• 遺伝子情報: human ... GPC2(221914)

詳細

特異性

Clone CT3 is a mouse monoclonal antibody that detects Glypican-2 (GPC2) and its glycated forms. It targets an epitope within the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 50 amino acids from the C-terminal region of human Glypican-2 (GPC2).

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Glypican-2 in SH-SY5Y cell lysate.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Glypican-2 in IMR-32 cell lysate.

Immunohistochemistry Applications: A representative lot detected Glypican-2 in Immunohistochemistry applications (Li, N., et al. (2021). Cell Rep Med. 2(6): 100297; Li, N., et al. (2020). J Histochem Cytochem. 68(12): 841-862).

ELISA: A representative lot detected Glypican-2 in ELISA applications (Li, N., et al. (2021). Cell Rep Med. 2(6): 100297).

Flow Cytometry Analysis: A representative lot detected Glypican-2 in Flow Cytometry applications (Li, N., et al. (2021). Cell Rep Med. 2(6): 100297; Li, N., et al. (2020). J Histochem Cytochem. 68(12): 841-862).

Western Blotting Analysis: A representative lot detected Glypican-2 in Western Blotting applications (Li, N., et al. (2021). Cell Rep Med. 2(6):100297).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

Glypican-2 (UniProt: Q8N158; also known as GPC2) is encoded by the GPC2 gene (Gene ID: 221914) in human. Glypicans belong to a family of heparan sulfate proteoglycans that are attached to the cell membrane via a GPI-anchor. They interact with multiple ligands, including morphogens, growth factors, chemokines, ligands, receptors, and components of the extracellular matrix through their heparan sulfate chains and core protein. GPC2 is synthesized with a signal sequence (aa 1-23), which is subsequently cleaved off to produce the mature GPC2 (aa 24-554). It also contains a propeptide sequence (aa 555-579) that is removed in the mature form. Glypicans are reported to regulate Wnt/β-catenin signaling and affect cell growth and proliferation. Overexpression of GPC2 is reported in several pediatric cancers. Its higher expression is also shown in neuroblastoma compared to the normal peripheral nerve tissue. GPC2 can modulate Wnt signaling and regulate MYCN in neuroblastoma pathogenesis. Genetically silencing of CPC2 is shown to inhibit neuroblastoma cell growth by suppressing N-Myc expression. Clone CT3 specifically binds to GPC2 in human cancer cells and does not react with normal tissues. It recognizes tumor-associated exons 3 and 10 of GPC2. (Ref.: Li, N., et al. (2020). J. Histochem. Cytochem. 68(12); 841-862; Li, N., et al., 2021, Cell Rep. Medicine 2, 100297).

物理的形状

Purified mouse monoclonal antibody IgG1 in PBS without azide.

再構成

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable