MAB2058Z

Anti-Integrin β4 Antibody, clone ASC-3, azide free

clone ASC-3, Chemicon^®, from mouse

• 別名: CD104, MAB2058
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: ASC-3, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Chemicon^®
• テクニック: flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NM_000213.3; NM_001005619.1; NM_001005731.1
• UniProtアクセッション番号: P16144
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ITGB4(3691)

詳細

特異性

Reacts with Human beta4 integrin (CD104).

免疫原

Human squamous cell carcinoma cells (SCC9)

アプリケーション

Research Category
細胞骨格

Research Sub Category
インテグリン

Anti-Integrin β4 Antibody, clone ASC-3, azide free detects level of Integrin β4 & has been published & validated for use in FC, IH & IP.

Inhibition of cell attachment: inhibits SCC9 cell adhesion to human laminin and laminin GD-2 peptide, but not to EHS laminin, type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen. Immunohistochemistry: on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. Flow cytometry: stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets. Immunoprecipitation: precipitates PAGE bands of 66, 76, 120, 135 and 200kDa (reduced) or 66, 110, 125, 130 and 190kDa (non-reduced) from surface-biotinylated SCC9 cells. Working dilutions must be determined by end user.

物理的形状

Protein A purified

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, containing no preservatives.

保管および安定性

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

アナリシスノート

Control
A431 cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable