MAB1946-IM

Anti-Nidogen 1 Antibody, clone ELM1

clone ELM1, from rat

• 別名: Nidogen-1, NID-1, Entactin
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

特性

• 由来生物: rat
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: ELM1, monoclonal
• 化学種の反応性: mouse
• 包装: antibody small pack of 25 μL
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_035047
• UniProtアクセッション番号: P10493
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Nid1(18073)

詳細

詳細

Nidogen-1 (UniProt: P10493; also known as NID-1, Entactin) is encoded by the Nid1 (also known as Ent) gene (Gene ID: 18073) ins murine species. Nidogen-1 is a highly conserved sulfated monomeric glycoprotein that is widely distributed in basement membranes and tightly associates with laminin. It is post-translationally N- and O-glycosylated. It is synthesized with a 28 aa signal peptide that is subsequently cleaved to produce the mature form. Nidogen -1 contains six EGF-like domains, four LDL-receptor class B repeats, one NIDO domain, one nidogen G2 barrel domain, and one thyroglobulin type 1 domain. Nidogen-1 shares about 50% homology with Nidigen-2 and shares similar adhesive properties. There is also a high degree of homology between murine and human Nidogen-1. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation.

特異性

Clone ELM1 is a rat monoclonal antibody that detects Nidogen-1 in murine species.

免疫原

Purified protein from basement membranes of mouse Engelbreth-Holm-Swarm (EHS) tumor.

アプリケーション

Anti-Nidogen 1, clone ELM1, Cat. No. MAB1946-I, is a rat monoclonal antibodythat detects Nidogen-1 and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Western Blotting Analysis: A representative lot detected Nidogen-1 in Western Blotting applications (Arends, F., et. al. (2015). PLoS One. 10(2):e0118090).

Immunocytochemistry Analysis: A representative lot detected Nidogen-1 in Immunocytochemistry applications (Kick, K., et. al. (2016). Arterioscler Thromb Vasc Biol. 36(12):2346-2357).

Immunohistochemistry Analysis: A representative lot detected Nidogen-1 in Immunohistochemistry applications (Chang, Y.T., et. al. (2016). J Clin Invest. 126(2):721-31).

品質

Evaluated by Immunohistochemistry in mouse kidney.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Nidogen-1 in mouse kidney tissue.

ターゲットの説明

136.54 kDa calculated.

物理的形状

Format: Purified

その他情報

Concentration: Please refer to lot specific datasheet.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1