MAB1120

Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31

clone 24-31, Chemicon^®, from mouse

• 別名: IGF-IR, CD221
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 24-31, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Chemicon^®
• テクニック: immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NM_000875.3
• UniProtアクセッション番号: P08069
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... IGF1R(3480)

詳細

特異性

Recognizes the alpha-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 283-440 (exon 4-6) of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The a-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. It is assumed that, as for the insulin receptor, the tyrosine kinase activity is essential for IGF-1 action.

Weakly reacts with Rabbit.

免疫原

IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
インスリン/エネルギーシグナル伝達

Acts as a Weak IGF-like Agonist in Bioassays (Thymidine incorporation into DNA)

Immunoprecipitation (Use Protein G): 2μg antibody/mg protein lysate.

Immunohistochemistry (Frozen and formalin/paraffin): Use antibody at 2-4ug/mL for 30 min. at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min. followed by cooling at room temperature for 20 minutes. Please note that EDTA is better than citrate for epitope unmasking.

Optimal working dilutions must be determined by end user.

Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31 detects level of Insulin-like Growth Factor-1 Receptor & has been published & validated for use in IH(P) & IP.

物理的形状

Format: Purified

Purified from ascites fluid by Protein G chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

保管および安定性

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable