CBA415
Live Dead Cell Viability Assay Kit for 3D and 2D cell culture
Live-dead cell viability kit for 3D and 2D cell cultures. Indicates live, dead and total cell count. Sufficient for 5 x 24-well plates or 12 x 96-well plates.
別名:
Viability Assay Kit
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About This Item
UNSPSCコード:
12352207
NACRES:
NA.84
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検出方法
fluorometric
詳細
The Live-Dead Cell Viability Assay Kit is a quick and simple three-color assay to measure cell viability. The kit consists of Calcein-AM (stains live cells), Propidium Iodide (stains dead cells) and Hoechst 33342 (stains all cells). The kit has been optimized for 3D cell culture (spheroids, human organoids and 3D matrices) and 2D cell culture (multiple cell types). The kit can be used for flow cytometry, fluorescence microscopy and with fluorescence microplate readers. The kit determines viability of cells based on plasma membrane integrity and esterase activity.
One kit contains reagents sufficient for 5 x 24-well plates or 12 x 96-well plates.
<bold>Advantages:</bold>
1. Tested and optimized for 3D cell culture, Spheroid culture, organoid culture, 2D cell culture and flow cytometry.
2. Easy to use and quick protocol.
3. Stronger intensity of Calcein AM and Hoechst dyes compared to other products.
Spectral Properties
Calcein AM: Ex/Em: 490/515 nm (Green)
Propidium Iodide: Ex/Em: 535/617 nm (Red)
Hoechst 33342: Ex/Em: 361/486 nm (Blue)
Protocols:
Protocols for staining 2D monolayer cell culture, 3D cell culture, 3D spheroids and 3D organoids are included in the datasheet.
One kit contains reagents sufficient for 5 x 24-well plates or 12 x 96-well plates.
<bold>Advantages:</bold>
1. Tested and optimized for 3D cell culture, Spheroid culture, organoid culture, 2D cell culture and flow cytometry.
2. Easy to use and quick protocol.
3. Stronger intensity of Calcein AM and Hoechst dyes compared to other products.
Spectral Properties
Calcein AM: Ex/Em: 490/515 nm (Green)
Propidium Iodide: Ex/Em: 535/617 nm (Red)
Hoechst 33342: Ex/Em: 361/486 nm (Blue)
Protocols:
Protocols for staining 2D monolayer cell culture, 3D cell culture, 3D spheroids and 3D organoids are included in the datasheet.
保管および安定性
Store at -20oC or below, and protect from light. The vials can be frozen and thawed upto 10 times. Before use, allow the vials to thaw to room temperature and centrifuge briefly.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
10 - Combustible liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
CBA415:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.
オルガノイド培養に関するFAQ3D細胞培養プロトコルに従ったオルガノイドの培養・分析方法をご覧ください。ヒントとコツ。
関連コンテンツ
Monitor barrier formation using colon PDOs, iPSC-derived colon organoids, Millicell® cell culture inserts, and the Millicell® ERS. 3.0.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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