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詳細
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
The reagent is an anti-mouse antibody F(ab′)2 fragment from goat. The F(ab′)2 fragments are obtained by pepsin cleavage. The fragments are conjugated to fluorescein isothiocyanate and stabilized in buffer. The molar ratio of fluorescein: protein is: 3 - 7.
PURIFICATION: The goat-lgG was purified by affinity chromatography and absorbed to remove cross-reactivity to human immunoglobulins.
PURIFICATION: The goat-lgG was purified by affinity chromatography and absorbed to remove cross-reactivity to human immunoglobulins.
特異性
Specific for mouse IgG, heavy and light chain. The cross-reactivities of the anti-mouse IgG antibody were tested in an ELISA. Minimum cross-reactivity to human IgG.
アプリケーション
Research Category
二次抗体及びコントロール抗体
二次抗体及びコントロール抗体
Research Sub Category
フラグメント特異的二次抗体
フラグメント特異的二次抗体
Immunohistochemistry: 1:200-1:500 (Coligan et al. 1997; Harlow & Lane 1988; Bullock & Petrusz 1982; Javois 1994)
Immunocytochemistry: 1:200-1:500 (Coligan et al. 1997; Harlow & Lane 1988; Bullock & Petrusz 1982; Javois 1994)
Flow cytometery: 1 μg per 1 x 10E6 cells (Coligan et al. 1997; Harlow & Lane 1988; Javois 1994) FITC absorption peak is 488-492nm, its emission peak is 520nm.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:200-1:500 (Coligan et al. 1997; Harlow & Lane 1988; Bullock & Petrusz 1982; Javois 1994)
Flow cytometery: 1 μg per 1 x 10E6 cells (Coligan et al. 1997; Harlow & Lane 1988; Javois 1994) FITC absorption peak is 488-492nm, its emission peak is 520nm.
Optimal working dilutions must be determined by the end user.
This Goat anti-Mouse IgG Antibody, F(ab′)2, FITC conjugate is validated for use in IF for the detection of Mouse IgG.
物理的形状
ImmunoAffinity Purified
Liquid in 0.02M Phosphate Buffer. 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA and 0.01% Sodium azide.
保管および安定性
The antibody conjugate solution is stable at 2–8°C for 12 months. Do not store in a diluted format.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AQ303F:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Lijun Xin et al.
Aging cell, 14(6), 1122-1126 (2015-08-08)
Aging confers increased susceptibility to common pathogens including influenza A virus. Despite shared vulnerability to infection with advancing age in humans and rodents, the relatively long time required for immune senescence to take hold practically restricts the use of naturally
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Sanpui, P; Zheng, X; Loeb, JC; Bisesi, JH; Khan, IA; Afrooz, AR; Liu, K; Badireddy et al.
Particle and Fibre Toxicology null
Daniel Sobrido-Cameán et al.
The Journal of comparative neurology, 531(1), 58-88 (2022-09-24)
The expression of the corticotropin-releasing hormone (PmCRH) and the CRH-binding protein (PmCRHBP) mRNAs was studied by in situ hybridization in the brain of prolarvae, larvae, and adults of the sea lamprey Petromyzon marinus. We also generated an antibody against the
Ramón Anadón et al.
The Journal of comparative neurology, 532(2), e25590-e25590 (2024-02-09)
Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the central nervous system (CNS) of vertebrates. Immunohistochemical techniques with specific antibodies against GABA or against its synthesizing enzyme, glutamic acid decarboxylase (GAD) allowed characterizing GABAergic neurons and fibers in the
Antón Barreiro-Iglesias et al.
The Journal of comparative neurology, 525(17), 3683-3704 (2017-08-05)
We employed an anti-transducin antibody (Gαt-S), in combination with other markers, to characterize the Gαt-S-immunoreactive (ir) system in the CNS of the sea lamprey, Petromyzon marinus. Gαt-S immunoreactivity was observed in some neuronal populations and numerous fibers distributed throughout the
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