ABF1207

Anti-phospho-Syk (Tyr323)

from rabbit, purified by affinity chromatography

• 別名: Tyrosine-protein kinase Syk, Tyr323 phosphorylated, p72-Syk, Tyr323 phosphorylated, p72Syk, Tyr323 phosphorylated, Spleen tyrosine kinase, Tyr323 phosphorylated
• UNSPSCコード: 12352203
• eCl@ss: 32160702

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 交差性: human
• テクニック: western blot: suitable
• NCBIアクセッション番号: NP_003168
• UniProtアクセッション番号: P43405
• 輸送温度: ambient
• ターゲットの翻訳後修飾: phosphorylation (pTyr323)
• 遺伝子情報: human ... SYK(6850)

詳細

詳細

Tyrosine-protein kinase Syk (EC 2.7.10.2; UniProt P43405; also known as p72-Syk, p72Syk, Spleen tyrosine kinase) is encoded by the SYK gene (Gene ID 6850) in human. Spleen tyrosine kinase (Syk) is a non-receptor tyrosine kinase highly expressed by haematopoietic cells. Syk plays a crucial role not only in adaptive immune receptor signalling, but also in cellular adhesion, innate immune recognition, osteoclast maturation, platelet activation and vascular development. Syk adopts an autoinhibited conformation in its inactive state and is activated upon binding immunoreceptor tyrosine-based activation motifs (ITAMs) and phosphorylation of tyrosine residues in the two linker regions (a.a. 108-160 and 260-370) between SH1 (a.a. 15-107) and SH2 (a.a. 168-259), and between SH2 and the kinase domain (a.a. 371-631). Initial ITAM binding triggers rapid activation of Syk, whereas subsequent phosphorylation leads to prolonged activation. In additon, Syk can phosphorylate ITAMs and autophosphorylate its own link region tyrosines, providing a positive feedback loop following initial ITAM-mediated Syk activation. Syk also mediates signalling of receptors that do not contain an ITAM, including integrins and C-type lectins, via interaction with ITAM-containing adaptors, such as DAP12 (TYROBP) and the FcR γ-chain (FcRγ).

特異性

Target phosphotyrosine residue coresponds to pTyr323 of human Syk spliced isoform 1 and pTyr300 of human Syk spliced isoform 2. Sequence surrounding the target tyrosine is not well conserved in mouse and rat Syk.

免疫原

Epitope: pTyr323

KLH-conjugated linear peptide corresponding to a sequence surrounding phosphorylated Tyr323 of human Syk.

アプリケーション

Research Category
炎症及び免疫

This rabbit polyclonal Anti-phospho-Syk (Tyr323) antibody, Cat. No. ABF1207 is validated for use in Western Blotting for the detection of Syk Tyr323 phosphorylation.

品質

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Syk Tyr323 phosphorylation induction in 10 µg of lysate from hydrogen peroxide-treated Jurkat cells.

ターゲットの説明

~72/69 kDa observed. 72.07/69.51 kDa (human Syk isoform 1/2) calculated.

関連事項

Replaces: 07-915

物理的形状

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable