コンテンツへスキップ
Merck
すべての画像(1)

主要文書

安全性情報

ABE1856

Sigma-Aldrich

Anti-CEP152 Antibody, N-Term

from rabbit

別名:

Centrosomal protein of 152 kDa

ログイン組織・契約価格を表示する


About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
現在、価格および在庫状況を閲覧できません。

由来生物

rabbit

品質水準

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

polyclonal

交差性

human

テクニック

electron microscopy: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

アイソタイプ

IgG

NCBIアクセッション番号

UniProtアクセッション番号

輸送温度

ambient

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... CEP152(22995)

詳細

Centrosomal protein of 152 kDa (UniProt O94986; also known as Cep152) is encoded by the CEP152 (also known as KIAA0912) gene (Gene ID 22995) in human. The mammalian Asl (Asterless) ortholog Cep152 is a centrosomal protein (CEP) that plays an important role in centriole duplication and centriole-to-centrosome conversion. The centrosome is the main microtubule-organizing center essential spindle formation and chromosome segregation during mitosis. Centrosomes are composed of two orthogonally arranged centrioles surrounded by pericentriolar material (PCM). Centriole duplication begins by assembling a procentriole in G1/S phase, where centrosomal scaffolds Cep152 and Cep192 (Spd-2 ortholog) mediate the recruitment of polo kinase Plk4 to centrosomes. This is a key event that triggers the entire duplication process. New centrioles assemble perpendicularly to pre-existing ones in G1/S phase and elongate throughout S and G2 phase. The conversion of fully elongated daughter centrioles into centrosomes during mitotic progression requires sequential loading of Cep135, Cep295 (Ana1 analog) and Cep152 onto daughter centrioles.Cep152 is also an important regulator of genomic integrity and cellular response to DNA damage. Overexpression of CEP152 can drive amplification of centrioles. CEP152 gene mutations are identified among individuals with Seckel syndrome, a heterogeneous autosomal recessive disorder characterized by a proportionate short stature, severe microcephaly and mental retardation, and a typical ′bird-head′ facial appearance. Cep152 impairment in Seckel patients-derived cells leads to accumulation of genomic defects as a result of replicative stress through enhanced activation of ATM signaling and increased H2AX phosphorylation.

特異性

This polyclonal antibody specifically detects human CEP152 protein. It targets an epitope within the first 87 amino acids from the N-terminal region. Recognizes both long and short isoforms of Cep152.

免疫原

Epitope: N-terminus
His-tagged recombinat fragment corresponding to 87 amino acids from the N-terminal end of human Centrosomal protein of 152 kDa (CEP152).

アプリケーション

Research Category
エピジェネティクス及び核内機能分子
Anti-CEP152, N-Term, Cat. No. ABE1856, is a highly specific rabbit polyclonal antibody that targets Centrosomal protein of 152KDa and has been tested in Electron Microscopy, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Electron Microscopy Analysis: A representative lot detected CEP152 in U2OS cells (Sonnen, K.F., et. al. (2013). J cell Sci. 126(Pt 14):3223-33).

Western Blotting Analysis: A representative lot detected CEP152 in U2OS cells transfected with myc-Plk4 and HEK293T cells transfected with Flag-Cep152-2 (Sonnen, K.F., et. al. (2013). J cell Sci. 126(Pt 14):3223-33).

Immunocytochemistry Analysis: A representative lot detected CEP152, N-Term in U2OS cells (Sonnen, K.F., et. al. (2013). J cell Sci. 126(Pt 14):3223-33;Greitzer-Antes, D., et. al. (2013). J cell Sci. 126(Pt 13):2914-23).

Immunoprecipitation Analysis: A representative lot detected CEP152, N-Term in HEK293T cells transfected with Flag-Cep152-2 (Sonnen, K.F., et. al. (2013). J cell Sci. 126(Pt 14):3223-33).

品質

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CEP152 in 10 µg of U2OS cell lysate.

ターゲットの説明

~230 kDa observed; 195.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein A purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

適切な製品が見つかりませんか。  

製品選択ツール.をお試しください

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

ABE1856:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Fatma Uzbas et al.
Bio-protocol, 13(17), e4812-e4812 (2023-09-20)
The centrosome governs many pan-cellular processes including cell division, migration, and cilium formation. However, very little is known about its cell type-specific protein composition and the sub-organellar domains where these protein interactions take place. Here, we outline a protocol for

質問

レビュー

評価値なし

アクティブなフィルタ

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら(テクニカルサービス)