AB6016

Anti-F-actin-capping protein subunit alpha-1 Antibody

from rabbit, purified by affinity chromatography

• 別名: capping protein (actin filament) muscle Z-line, alpha 1, CapZ alpha-1, F-actin-capping protein subunit alpha-1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: pig, mouse, human, rat
• 化学種の反応性（ホモロジーによる予測）: rhesus macaque (based on 100% sequence homology), bovine (based on 100% sequence homology), primate (based on 100% sequence homology), canine (based on 100% sequence homology), porcine (based on 100% sequence homology)
• テクニック: immunocytochemistry: suitable; western blot: suitable
• UniProtアクセッション番号: P52907
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: bovine ... Capza1(534656) ; dog ... Capza1(475857) ; human ... CAPZA1(829) ; mouse ... Capza1(12340) ; pig ... Capza1(100037957) ; rat ... Capza1(691149) ; rhesus monkey ... Capza1(705653)

詳細

詳細

F-actin capping protein subunit alpha (CapZ alpha) belongs to the F-actin capping protein family, which are characteristic heterodimers, consisting of an α subunit (31-36 kDa) and a β subunit (28-32 kDa), that cap the barbed end of actin filaments within all eukaryotes. Their ability to bind the actin filaments is in a manner independent of Ca2++ and requires the C-terminal end of both subunits for optimal binding. CapZ is contained within the Z-discs of striated muscle, where it functions to inhibit the polymerization and depolymerization of actin. Within vertebrates, there are three isoforms for each of CapZ’s subunits. All three alpha subunit isoforms are components of the WASH complex. Little is known about isoforms 1 and 2; however, isoform 3 is thought to be involved in spermatid morphogenesis and is exclusively observed within sperm and testis, with highest expression within the neck region of sperm.

免疫原

GST-tagged recombinant protein corres-ponding to human F-actin-capping protein subunit alpha-1.

アプリケーション

Research Sub Category
細胞骨格

Detect F-actin-capping protein subunit alpha-1 using this Anti-F-actin-capping protein subunit alpha-1 Antibody validated for use in WB & IC.

Research Category
Cell Structure

Western Blot (Snap i.d.) Analysis: 1 µg/mL from a previous lot detected F-actin-capping protein subunit alpha-1 on 10 µg of HeLa cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected F-actin-capping protein subunit alpha-1 in NIH/3T3, HeLa, and A431 cells.

品質

Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected F-actin-capping protein subunit alpha-1 on 10 µg of HeLa cell lysate.

ターゲットの説明

~35 kDa

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
HeLa cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable