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由来生物
rabbit
抗体製品の状態
affinity purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
rat, mouse
メーカー／製品名
Chemicon®
テクニック
immunohistochemistry: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... AQP3(360)
特異性
Recognizes Aquaporin 3.
SPECIES REACTIVITIES: The epitope specific for Aquaporin 3 is highly homologous in human and sheep (15/18). Reactivity with other species has not yet been tested.
SPECIES REACTIVITIES: The epitope specific for Aquaporin 3 is highly homologous in human and sheep (15/18). Reactivity with other species has not yet been tested.
免疫原
Highly purified peptide corresponding to residues 275-292 of rat Aquaporin 3 (Accession Number P47862) coupled to KLH.
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
イオンチャネル及びトランスポーター
イオンチャネル及びトランスポーター
Anti-Aquaporin 3 Antibody detects level of Aquaporin 3 & has been published & validated for use in WB, IH.
Western blotting: 1:200 on rat kidney membranes. Identifies a 31kDa and 40kDa band. The large band may represent the glycosylated form of the protein.
Immunohistochemistry on mouse kidney sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on mouse kidney sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
物理的形状
Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.025% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
保管および安定性
Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
アナリシスノート
Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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危険有害性情報
注意書き
危険有害性の分類
Aquatic Chronic 3
保管分類コード
13 - Non Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Takashi Nagai et al.
Neurourology and urodynamics, 38(6), 1560-1570 (2019-06-14)
To measure the effects of nicotine on lower urinary tract symptoms (LUTS), bladder blood flow, and the urothelial markers hypoxia-inducible factor 1α (HIF1α), uroplakin III (UPIII), and aquaporin 3 (AQP3). Ten-week-old female Sprague Dawley rats were subcutaneously injected with 2 mg/kg
Jae Mi Suh et al.
Molecular endocrinology (Baltimore, Md.), 20(12), 3412-3420 (2006-09-16)
Nuclear receptors are transcriptional regulators that play important roles in embryonic development and organogenesis. To study the potential roles of nuclear receptors in kidney development, we examined the expression patterns of a subset of nuclear receptors in which specific antibodies
Harumi Hisaki et al.
American journal of physiology. Renal physiology, 303(7), F1049-F1059 (2012-07-27)
Saposin D-deficient (Sap-D(-/-)) mice develop polydipsia/polyuria and die prematurely due to renal failure with robust hydronephrosis. Such symptoms emerged when they were around 3 mo of age. To investigate the pathogenesis of their water mishandling, we attempted to limit water
Kyungmi Yang et al.
Marine drugs, 18(8) (2020-07-28)
Radiation dermatitis (RD) is one of the most common side effects of radiotherapy; its symptoms progress from erythema to dry and moist desquamation, leading to the deterioration of the patients' quality of life. Active metabolites in brown seaweed, including phlorotannins
Aquaporin3 is a sperm water channel essential for postcopulatory sperm osmoadaptation and migration.
Qi Chen et al.
Cell research, 21(6), 922-933 (2010-12-08)
In the journey from the male to female reproductive tract, mammalian sperm experience a natural osmotic decrease (e.g., in mouse, from ~415 mOsm in the cauda epididymis to ~310 mOsm in the uterine cavity). Sperm have evolved to utilize this
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