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由来生物
rabbit
品質水準
抗体製品の状態
affinity purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
mouse
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
mouse ... Fn1(14268)
詳細
Fibronectin is a high molecular weight extracellular adhesion molecule involved in many cellular processes, including tissue repair or wound healing, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two major forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix (ECM), and 2) as a soluble disulfide-linked dimer found in the plasma. The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. It can also serve to organize cellular interaction with the ECM by binding to different components of the ECM and to membrane-bound fibronectin receptors on cell surfaces. The immunogen for fibronectin polyclonal antibody AB2033 is purified mouse plasma. The antibody shows less than 0.1% reactivity with mouse laminin, and collagen types I, III, and IV by radioimmunoassay.
特異性
This antibody reacts with mouse fibronectin. Shows less than 0.1% reactivity with mouse Laminin, and Collagen Types I, III, and IV by radioimmunoassay.
免疫原
Fibronectin extracted and purified from mouse plasma.
アプリケーション
Research Category
細胞骨格
細胞骨格
Research Sub Category
ECMタンパク質
ECMタンパク質
Detect Fibronectin using this Anti-Fibronectin Antibody validated for use in ELISA, IC, IH, RIA.
Immunohistochemistry:
1:80 dilution of a previous lot was used in immunofluorescent staining of frozen mouse skin and liver tissues.
Radioimmunoassay:
A previous lot of this antibody was used in RIA.
ELISA:
A 1:200 dilution of a previous lot was used in ELISA.
Optimal working dilutions must be determined by the end user.
1:80 dilution of a previous lot was used in immunofluorescent staining of frozen mouse skin and liver tissues.
Radioimmunoassay:
A previous lot of this antibody was used in RIA.
ELISA:
A 1:200 dilution of a previous lot was used in ELISA.
Optimal working dilutions must be determined by the end user.
品質
Evaluated by immunocytochemistry on NIH/3T3 cells.
Immunocytochemistry:
Representative image from a previous lot.
Confocal fluorescent analysis of NIH/3T3 using AB2033 rabbit polyclonal antibody (Red). Actin filaments have been labeled with AlexaFluor 488 -Phalloidin (Green). Nucleus is stained with DAPI (Blue).
Immunocytochemistry:
Representative image from a previous lot.
Confocal fluorescent analysis of NIH/3T3 using AB2033 rabbit polyclonal antibody (Red). Actin filaments have been labeled with AlexaFluor 488 -Phalloidin (Green). Nucleus is stained with DAPI (Blue).
物理的形状
ImmunoAffinity Purified
The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µl/ml of antibiotics and antimycotics
保管および安定性
Stable for 1 year at -20ºC from date of receipt.
アナリシスノート
Control
Extracellular matrix in most tissue
Extracellular matrix in most tissue
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB2033:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Laetitia Sabatier et al.
The Biochemical journal, 456(2), 283-295 (2013-09-28)
Fibrillins constitute the backbone of extracellular multifunctional assemblies present in elastic and non-elastic matrices, termed microfibrils. Assembly of fibrillins into microfibrils and their homoeostasis is poorly understood and is often compromised in connective tissue disorders such as Marfan syndrome and
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Kidney international, 86(2), 303-315 (2014-02-21)
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Nephrology, Dialysis, and Transplantation null
Katsutoshi Sekine et al.
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Birthdate-dependent neuronal layering is fundamental to neocortical functions. The extracellular protein Reelin is essential for the establishment of the eventual neuronal alignments. Although this Reelin-dependent neuronal layering is mainly established by the final neuronal migration step called "terminal translocation" beneath
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Hanson, KP; Jung, JP; Tran, QA; Hsu, SP; Iida, R; Ajeti, V; Campagnola, PJ; Eliceiri et al.
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