価格・在庫情報は確認できません。詳細については、Merckをご覧ください。
おすすめの製品
由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
交差性
human, mouse, rat
メーカー／製品名
Chemicon®
テクニック
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ITGB4(3691)
mouse ... Itgb4(192897)
rat ... Itgb4(25724)
特異性
Specific for beta4 integrin as determined by immunoprecipitation. The epitope recognized is on the cytoplasmic domain of the protein and is thus intracellular. No cross-reactivity to other integrin b subunits.
免疫原
Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human β4 integrin subunit (Tamura et al. 1990). The sequence used is NH2-(K)GTLSTHMDQQFFQT-amide. The lysine (K) is not in the β4 sequence and was added to conjugate the peptid
アプリケーション
Anti-Integrin β4 Antibody is an antibody against Integrin β4 for use in IP, IH.
Immunoprecipitation from labeled cell extracts: 5 μL of serum is sufficient to precipitate alpha6beta4 from 1-2 x 10E6 cells. In some cases immunoprecipitation from 35S methionine labeled cells results in two bands of 95 and 55 kDa of unknown origin in addition to the 200 kDa and 140 kDa bands corresponding to beta4 and alpha6.
Immunohistochemistry: 5 μg/mL. See protocol below.
Optimal working dilutions must be determined by the end user.
Suggested Protocol for Immunohistochemical:
1. Fix cryostat sections with a 1:1 chloroform-acetone mixture for 10 minutes at room temperature.
2. Air Dry.
3. Wash with 2 mL of 1x PBS.
4. Add 200 μL of first antibody (5 μg/mL) to each slide. Incubate for 1 hour at room temperature in a humidity chamber.
5. Wash with 5 mL of 1x PBS.
6. Add appropriately diluted anti-rabbit secondary antibody conjugate and incubate for 1 hour in a humidity chamber
7. Wash with 5 mL of 1x PBS.
8. Add 200 μL of development solution (containing DAB or other substrate depending on the secondary antibody) and incubate at room temperature for the necessary time to develop the dark color. Follow the reaction with a microscope, to establish the correct color intensity.
9. Block the reaction by washing with 2 mL of 1x PBS.
10. Counterstain with hematoxylin as desired.
Immunohistochemistry: 5 μg/mL. See protocol below.
Optimal working dilutions must be determined by the end user.
Suggested Protocol for Immunohistochemical:
1. Fix cryostat sections with a 1:1 chloroform-acetone mixture for 10 minutes at room temperature.
2. Air Dry.
3. Wash with 2 mL of 1x PBS.
4. Add 200 μL of first antibody (5 μg/mL) to each slide. Incubate for 1 hour at room temperature in a humidity chamber.
5. Wash with 5 mL of 1x PBS.
6. Add appropriately diluted anti-rabbit secondary antibody conjugate and incubate for 1 hour in a humidity chamber
7. Wash with 5 mL of 1x PBS.
8. Add 200 μL of development solution (containing DAB or other substrate depending on the secondary antibody) and incubate at room temperature for the necessary time to develop the dark color. Follow the reaction with a microscope, to establish the correct color intensity.
9. Block the reaction by washing with 2 mL of 1x PBS.
10. Counterstain with hematoxylin as desired.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
10 - Combustible liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB1922:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Palmitoylation by DHHC3 is critical for the function, expression, and stability of integrin ?6?4.
Sharma, C; Rabinovitz, I; Hemler, ME
Cellular and Molecular Life Sciences null
Haiyao Wang et al.
The Journal of biological chemistry, 290(43), 26103-26113 (2015-09-10)
The α6β4 integrin is known to associate with receptor tyrosine kinases when engaged in epithelial wound healing and in carcinoma invasion and survival. Prior work has shown that HER2 associates with α6β4 integrin and syndecan-1 (Sdc1), in which Sdc1 engages
Haiyao Wang et al.
The Journal of biological chemistry, 289(44), 30318-30332 (2014-09-10)
Epithelial cells are highly dependent during wound healing and tumorigenesis on the α6β4 integrin and its association with receptor tyrosine kinases. Previous work showed that phosphorylation of the β4 subunit upon matrix engagement depends on the matrix receptor syndecan (Sdc)-1
K Triantafilou et al.
Journal of virology, 74(13), 5856-5862 (2000-06-14)
Human parechovirus 1 (HPEV1) displays an arginine-glycine-aspartic acid (RGD) motif in the VP1 capsid protein, suggesting integrins as candidate receptors for HPEV1. A panel of monoclonal antibodies (MAbs) specific for integrins alphavbeta3, alphavbeta1, and alphavbeta5, which have the ability to
Human umbilical cord mesenchymal stromal cells exhibit immature nucleus pulposus cell phenotype in a laminin-rich pseudo-three-dimensional culture system.
Chon, BH; Lee, EJ; Jing, L; Setton, LA; Chen, J
Stem Cell Research & Therapy null
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）
