AB1510

Anti-Beta (β)-Amyloid antibody

CHEMICON^®, rabbit polyclonal

• 別名: Beta-APP, A-beta
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 製品名: Anti-Amyloid β Antibody, aa1-14 h β-amyloid, serum, Chemicon^®
• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 交差性: human
• メーカー／製品名: Chemicon^®
• テクニック: immunohistochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NM_000484.2; NM_201413.1; NM_201414.1
• UniProtアクセッション番号: P05067
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... APP(351)

詳細

特異性

Beta amyloid. Sequence is contained in the Amyloid beta-A4 protein amino acids 672-685 of human APP. The sequence is identical in the following other related proteins:

Cerebral vascular amyloid peptide) (CVAP) (Protease nexin-II) (PN-II) (APPI) (PreA4) [Contains: Soluble APP-alpha (S-APP-alpha); Soluble APP-beta (S-APP-beta); C99; Beta-amyloid protein 42 (Beta-APP42); Beta-amyloid protein 40 (Beta-APP40); C83; P3(42); P3(40); Gamma-CTF(59) (Gamma-secretase C-terminal fragment 59) (Amyloid intracellular domain 59) (AID(59)); Gamma-CTF(57) (Gamma-secretase C-terminal fragment 57).

100% homology in human, pig, sheep, rabbit, bovine, 78% identities (11/14), with 12/14 positives (85%) in rat and mouse beta amyloid containing proteins.

免疫原

Epitope: aa1-14 h beta-amyloid

Synthetic peptide that corresponds to the first 14 amino acids of the N-terminal sequence of beta-amyloid. {DAEFRHDSGYEVHH}

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経変性疾患

Anti-Amyloid Antibody, β, aa1-14 h β-amyloid detects level of Amyloid & has been published & validated for use in WB, IH.

Immunohistochemistry on formic acid treated histological sections. Suggested working dilution is >1:200. AB1510 will detect both cerebrovascular and core amyloid. Formic acid pretreatment (Vinters et al., 1988) involves deparaffinizing sections, follwed by incubation in a 99% formic acid solution (10-20 min), a tap water rinse (10 min), 0.3% H202 in tris-buffered saline, then 3.0% NGS (30 min) followed by primary antibody.

Immunoblotting of formic acid extracts of brain tissue.

ELISA, direct:≥ 1:1000

Optimal working dilutions must be determined by end user.

物理的形状

Rabbit serum with 0.01% thimerosal.

保管および安定性

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable