616407

TACE Substrate IV, Fluorogenic

• 別名: TACE Substrate IV, Fluorogenic, TNF-α Convertase Enzyme Substrate IV, Fluorogenic, Abz-LAQAVRSSSR-Dpa, ADAM-17 Substrate IV, Fluorogenic
• 実験式（ヒル表記法）: C₅₉H₉₃N₂₃O₂₀
• 分子量: 1444.51
• UNSPSCコード: 12352200
• NACRES: NA.77

特性

• アッセイ: ≥95% (HPLC)
• 品質水準: 100
• 形状: lyophilized solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated; protect from light
• 色: yellow
• 溶解性: DMSO: 5 mg/mL
• 蛍光検出: λ_ex ~320 nm; λ_em ~420 nm
• 輸送温度: ambient
• 保管温度: 2-8°C

詳細

詳細

An internally quenched, fluorogenic, 10-amino acid peptide substrate that is useful for monitoring TNF-α Convertase Enzyme (TACE) activity by a continuous fluorometric assay. Also useful for screening TACE inhibitors (k_cat = 21.6 s^-1 when the substrate concentration is half the V_max, S_0.5 = 342 µM). The substrate is cleaved at the Ala-Val amide bond. The substrate is not substantially cleaved by MMP-1, MMP-9, or MMP-13. Displays improved solubility in aqueous buffers. Excitation max: ~320 nm; Emission max: ~420 nm.

An internally-quenched fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity and high-throughput screening of TACE inhibitors (k_cat = 21.6 s^-1 and substrate concentration at half the V_max, S_0.5 = 342 µM). The substrate is cleaved at Ala-Val amide bond. Acts as a poor substrate for MMP-1, MMP-9, and MMP-13. Displays better solubility in aqueous buffers.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
Fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity

Product does not compete with ATP.

Reversible: no

kcat = 21.6 s-1 and substrate concentration at half the Vmax, S0.5 = 342 µM

警告

Toxicity: Standard Handling (A)

シーケンス

Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa

物理的形状

Supplied as a trifluoroacetate salt.

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

その他情報

Jin, G., et al. 2002. Anal. Biochem.302, 269.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable