521225

PDGF, BB Homodimer, Human, Recombinant, E. coli

• 別名: rhPDGF-BB
• UNSPSCコード: 12352202

特性

• アッセイ: ≥95% (SDS-PAGE)
• 品質水準: 100
• フォーム: lyophilized
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 不純物: ≤1 ng/μg Endotoxin (ng/μg PDGF-BB)
• 輸送温度: dry ice
• 保管温度: −70°C

詳細

詳細

Recombinant, human platelet-derived growth factor (disulfide-linked dimer of two 109 amino acid B-chain monomers) expressed in E. coli. Shown to be a potent chemoattractant for neutrophils, mesenchymal and mononuclear cells. Has the same amino acid sequence as mature human PDGF-B. Rapidly activates protein kinase D in vascular smooth muscle cells.

Recombinant, human platelet-derived growth factor (disulfide-linked dimer of two 109 amino acid B-chain monomers) expressed in E. coli. Shown to be a potent chemoattractant for neutrophils, mesenchymal, and mononuclear cells. PDGF represents one of the major mitogens present in platelets. It exerts its effects by binding to specific high-affinity dimeric receptor tyrosine kinases. Material has the same amino acid sequence as mature human PDGF-B. Two distinct PDGF receptors have been identified: the PDGFα receptor, which binds to all three forms of PDGF, and the PDGFβ receptor, which can bind only PDGF-BB and PDGF-AB.

生物化学的／生理学的作用

ED₅₀ = 1-5 ng/ml as determined by the dose dependent proliferation of BALB/c 3T3 cells

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from a sterile-filtered solution, carrier free.

再構成

Reconstitute to a concentration of ≥100 µg/ml 100 mM acetic acid, 0.1% BSA. Further dilute with low endotoxin medium or buffered solution containing FBS or tissue culture grade BSA just prior to use.

その他情報

Abedi, H., et al. 1998. FEBS Lett. 427, 209.
Abboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
Westermark, B., and Heldin, C.-H. 1991. Cancer Res.51, 5087.
Raines, E.W., et al. 1985. Methods Enzymol.109, 749.
Johnson, A., et al. 1984. EMBO J.3, 921.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable