5.04843

Fbxo3 Inhibitor, BC-1215

• 別名: Fbxo3 Inhibitor, BC-1215, N¹,N²- bis(4-(Pyridin-2-yl)benzyl)ethane-1,2-diamine, F Box Only Protein 3 Inhibitor, F Box Protein 3 Inhibitor, BC1215, F Box and Leucine-rich Repeat Protein 2 Activator, Fbxl2 Activator
• 実験式（ヒル表記法）: C₂₆H₂₆N₄
• CAS番号: 1507370-20-8
• 分子量: 394.51
• MDL番号: MFCD28118991
• UNSPSCコード: 51111800
• NACRES: NA.77

特性

• アッセイ: ≥98% (HPLC)
• 品質水準: 100
• フォーム: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: beige
• 溶解性: DMSO: 10 mg/mL
• 保管温度: −20°C
• SMILES記法: N(CCNCc3ccc(cc3)c4ncccc4)Cc1ccc(cc1)c2ncccc2
• InChI Key: IXEPQJQQSLMESJ-UHFFFAOYSA-N

詳細

詳細

A cell-permeable, bis-pyridinylbenzylethanamine that disrupts Fbxo3-Fbxl2 interaction and effectively prevents SCF-Fbxo3-catalyzed Fbxl2 ubiquitination, resulting in cellular Fbxl2 upregulation and thereby SCF-Fbxl2-catalyzed TRAFs (TNF Receptor-Associated Factors) ubiquitination. Effectively reduces cellular TRAFs (5 to 127 µM) and prevents TRAF-mediated cytokines production from LPS-stimulated human PBMC (25 µM). Shown to greatly prevent Cecal Ligation & Puncture-induced plasma cytokine elevation and substantially reduce the severity of lung inflammation post intratracheal P. aeruginosa infection in mice (100 µg/mouse; i.p.) in vivo.

A cell-permeable, bis-pyridinylbenzylethanamine that disrupts Fbxo3-Fbxl2 interaction by targeting Fbxo3 C-terminal ApaG domain and effectively prevents SCF-Fbxo3-catalyzed Fbxl2 ubiquitination, resulting in upregulation of cellular Fbxl2 and thereby SCF-Fbxl2-catalyzed ubiquitination of TRAFs (TNF Receptor-Associated Factors). Effectively reduces cellular TRAFs in human macrophage U937, PBMC, and murine murine MLE cultures (16 to 18 h with 5 to 127 µM BC-1215), resulting in downregulation of TRAF-mediated cytokines production from LPS-stimulated human PBMC (2 µg/mL LPS; 25 µM BC-1215;16 h). Intraperitoneal injection in mice in vivo (100 µg/mouse) is shown to greatly prevent circulating proinflammatory cytokines increase post CLP (Cecal Ligation & Puncture) and substantially reduce the severity of cell infiltration in lung after intratracheal P. aeruginosa PA103 infection.

生物化学的／生理学的作用

Cell permeable: yes

Primary Target
Fbox3

Reversible: yes

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

その他情報

Chen, B.B., et al. 2013. Nat. Immunol.14, 470.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable