218829

Caspase-3 Substrate IX, Fluorogenic

A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photostable caspase-3 fluorogenic substrate.

• 別名: Caspase-3 Substrate IX, Fluorogenic, (Z-DEVD)₂-Rh 110
• 実験式（ヒル表記法）: C₇₂H₇₈N₁₀O₂₇
• 分子量: 1515.44
• UNSPSCコード: 12352200

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• 形状: lyophilized solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated; protect from light
• 色: yellow
• 溶解性: DMSO: 1 mg/mL
• 蛍光検出: λ_ex ~485 nm; λ_em ~535 nm
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photo-stable caspase-3 fluorogenic substrate. Reported to detect less than 1 ng/ml of caspase-3. Also reported to detect capase-3 activity in intact cells by flow cytometry. Useful for high throughput screening applications. Excitation max: ~485 nm; emission max: ~535 nm.

A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photostable caspase-3 fluorogenic substrate. Reported to detect less than 1 ng/ml of caspase-3. Useful for high throughput screening applications.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
A highly sensitive, photostable caspase-3 fluorogenic substrate

Product does not compete with ATP.

Reversible: no

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

シーケンス

(Z-Asp-Glu-Val-Asp)₂-Rhodamine 110

物理的形状

Supplied as a trifluoroacetate salt.

再構成

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

その他情報

Hug, H., et al. 1999. Biochemistry38, 13906.
Liu, J., et al. 1999. Bioorg. Med. Chem. Lett.9, 3231.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable