218765

Caspase-9 Substrate I, Fluorogenic

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6).

• 別名: Caspase-9 Substrate I, Fluorogenic, Ac-LEHD-AFC
• 実験式（ヒル表記法）: C₃₃H₃₈F₃N₇O₁₁
• CAS番号: 210345-03-2
• 分子量: 765.69
• UNSPSCコード: 12352200
• NACRES: NA.77

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• 形状: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated; protect from light
• 色: white
• 溶解性: DMSO: 5 mg/mL
• 蛍光検出: λ_ex ~400 nm; λ_em ~505 nm
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). May also act as a substrate for caspase-4 and caspase-5. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).

生物化学的／生理学的作用

Cell permeable: no

Primary Target
A fluorogenic substrate for caspase-9

Product does not compete with ATP.

Reversible: no

警告

Toxicity: Standard Handling (A)

シーケンス

Ac-Leu-Glu-His-Asp-AFC

物理的形状

Supplied as a trifluoroacetate salt.

再構成

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

その他情報

Ito, A., et al. 1999. Biochim. Biophys. Acta 1452, 263.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable