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由来生物
human
品質水準
リコンビナント
expressed in baculovirus infected Sf21 cells
比活性
2337 U/mg
分子量
Mw 80.6 kDa
メーカー/製品名
Upstate®
テクニック
activity assay: suitable (kinase)
NCBIアクセッション番号
UniProtアクセッション番号
詳細
N-terminal GST- tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells.
Product Source: Recombinant protein expressed in Sf21 cells
アプリケーション
Research Category
代謝
代謝
Research Sub Category
心血管疾患
代謝障害
心血管疾患
代謝障害
生物化学的/生理学的作用
Protein Target: GCK
Target Sub-Family: STE
品質
Routinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active GCK.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active GCK.
単位の定義
Specific Activity: where one unit of GCK activity is defined as 1nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM.
物理的形状
Purified using glutathione-agarose. Purity 52% by SDS-PAGE and Coomassie blue staining.
保管および安定性
Stable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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