1.24546

Fluorescein-5-isothiocyanate (FITC)

for biochemistry and for fluorescence microscopy

• 別名: Fluorescein-5-isothiocyanate (FITC)
• 実験式（ヒル表記法）: C₂₁H₁₁NO₅S
• CAS番号: 3326-32-7
• 分子量: 389.38
• MDL番号: MFCD00005063
• UNSPSCコード: 12171501
• EC Index Number: 222-042-0

特性

• 品質水準: 400
• 形状: solid
• 組成: Dye, ≥95% spectrophotometric assay
• mp: >360 °C
• εmax: 2200-2380 at 488-494 nm in 0.1 M NaOH at 0.003 g/L
• アプリケーション: diagnostic assay manufacturing; hematology; histology
• 保管温度: 2-8°C
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI Key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

詳細

詳細

In immuno histology/ cytology, Fluorescein-5-isothiocyanate (FITC) for biochemistry and for fluorescence microscopy, serve as marker substance for the visualization and for the detection of tissue-bound bio molecules like e.g. antigens, lectins, various proteins, peptides, nucleic acids, oligo- and polysaccharides in samples of human origin. FITC-marked antibodies bind specifically to the appropriate target structures in the tissue. An optimum FITC-protein (antibody) ratio is important for a good result.
FITC is an IVD registered product and CE certified, thus can be used for clinical diagnostic purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

アナリシスノート

Dye content (spectrophotometrically): ≥ 95 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (Sodium hydroxide solution 0.1 mol/l): 488 - 494 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; NaOH 0.1 mol/l): 2200 - 2380
TLC-Test: passes test

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(℃): Not applicable