09-813
Anti-RING finger protein 8 Antibody
from rabbit, purified by affinity chromatography
別名:
ring finger protein 8, C3HC4-type zinc finger protein, ring finger protein (C3HC4 type) 8, UBC13/UEV-interacting ring finger protein, E3 ubiquitin-protein ligase RNF8
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About This Item
UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
価格・在庫情報は確認できません。詳細については、Merckをご覧ください。
由来生物
rabbit
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
交差性
human, mouse
交差性（ホモロジーによる予測）
chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
テクニック
immunocytochemistry: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... RNF8(9025)
詳細
RING Finger protein 8 (RNF8) is DNA binding protein belonging to the RING (Really Interesting New Gene) finger group of proteins. This family is characterized by a RING finger domain containing a Cys3HisCys4 amino acid motif capable of binding two zinc cations. RNF8 contains two domains of interest, the characteristic RING finger motif in the C-terminus and a FHA domain within the N-terminus, and is a critical E3 ubiquitin-protein ligase involved in the assembly of repair proteins to damaged DNA sites. After DNA double-strand breaks, RNF8 is recruited by ATM-phosphorylated MDC1 to the damage sites, where it functions to mediate H2A and H2AX ubiquitination, which in turn promotes TPR53BPI formation and the ionizing radiation-induced foci (IRIF) of BRCA1. RNF8 may also play regulatory roles in cytokinesis, correct exiting from mitosis, and RXRA-mediated transcriptional activity.
特異性
This antibody recognizes RING finger protein 8.
免疫原
Linear peptide corresponding to amino acids 135-148 of human RING finger protein 8.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン修飾タンパク質
細胞周期、DNA複製及び修復
ヒストン修飾タンパク質
細胞周期、DNA複製及び修復
Use Anti-RING finger protein 8 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect RING finger protein 8 also known as C3HC4-type zinc finger protein.
Western Blot (SNAP ID) Analysis: 1 µg/mL of a representative lot of this antibody detected RING finger protein 8 on 10 µg of U2OS nuclear extract.
Immunocytochemistry Analysis: 1:500 dilution of a representative lot this antibody detected RING finger protein 8 in NIH/3T3 and A431 cells.
Immunocytochemistry Analysis: 1:500 dilution of a representative lot this antibody detected RING finger protein 8 in NIH/3T3 and A431 cells.
品質
Evaluated by Western Blot in U2OS nuclear extract.
Western Blot Analysis: 0.1 µg/mL of this antibody detected RING finger protein 8 on 10 µg of U2OS nuclear extract.
Western Blot Analysis: 0.1 µg/mL of this antibody detected RING finger protein 8 on 10 µg of U2OS nuclear extract.
ターゲットの説明
~ 58 kDa observed. Two different isoforms have been described. Isoform 1: 55.5 kDa and Isoform 2: 11 kDa
物理的形状
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
アナリシスノート
Control
U2OS nuclear extract
U2OS nuclear extract
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
09-813:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Feng Zhao et al.
Oncology letters, 21(1), 42-42 (2020-12-03)
Breast cancer is one of the malignant tumors with the highest mortality rate. With the development of precise treatment technology for cancer, numerous molecular targets have been identified and applied in the treatment of diseases. The present study investigated the
John J Krais et al.
Cancer research, 80(13), 2848-2860 (2020-03-28)
BRCA1 gene mutations impair homologous recombination (HR) DNA repair, resulting in cellular senescence and embryonic lethality in mice. Therefore, BRCA1-deficient cancers require adaptations that prevent excessive genomic alterations from triggering cell death. RNF168-mediated ubiquitination of γH2AX at K13/15 (ub-H2AX) serves
Tingting Zhou et al.
Cell death & disease, 13(4), 352-352 (2022-04-17)
Androgen receptor (AR) signaling drives prostate cancer (PC) progression. Androgen deprivation therapy (ADT) is temporally effective, whereas drug resistance inevitably develops. Abnormal expression of AR/ARV7 (the most common AR splicing variant) is critical for endocrine resistance, while the detailed mechanism
Qinglei Hang et al.
Nature communications, 12(1), 4033-4033 (2021-07-01)
In response to DNA double-strand breaks (DSBs), repair proteins are recruited to the damaged sites. Ubiquitin signaling plays a critical role in coordinating protein recruitment during the DNA damage response. Here, we find that the microRNA biogenesis factor DGCR8 promotes
Keren Baranes-Bachar et al.
Molecular cell, 69(5), 866-878 (2018-03-03)
Double-strand breaks (DSBs) are critical DNA lesions that robustly activate the elaborate DNA damage response (DDR) network. We identified a critical player in DDR fine-tuning: the E3/E4 ubiquitin ligase UBE4A. UBE4A's recruitment to sites of DNA damage is dependent on
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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