bovine (based on 100% sequence homology), rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), zebrafish (based on 100% sequence homology)
テクニック
immunocytochemistry: suitable western blot: suitable
S6 (Ribosomal protein S6, RpS6, 40S ribosomal protein S6) is known to play a role in cell growth and proliferation. It lays downstream of both the PI3K/Akt/mTOR pathway as a substrate of p70 S6 kinase and the Ras/Erk pathway as a substrate of RSK. S6 is phosphorylated in response to stimulation by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. There are two major areas of S6 phosphorylation; Ser235/Ser236 that are known to be phosphorylated by both p70S6 Kinase as well as RSK and Ser240/244 which is thought to only be phosphorylated by p70S6K.
特異性
This antibody recognizes Ribosomal Protein S6 phosphorylated at Ser240/Ser244.
免疫原
Epitope: Phosphorylated Ser240/Ser244
KLH-conjugated linear peptide corresponding to human Ribosomal Protein S6 phosphorylated at Ser240/Ser244.
アプリケーション
Research Category 細胞シグナル伝達
Research Sub Category PI3K、Akt及びmTORシグナル伝達
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Ribosomal Protein S6 in A431 cells and PMA treated A431 cells.
This phospho-Ribosomal Protein S6 antibody is validated for use in WB & ICC for the detection of the phospho-Ribosomal Protein S6 protein.
品質
Evaluated by Western Blot in PMA treated and untreated A431 cell lysates.
Western Blot Analysis: 2 µg/mL of this antibody detected Ribosomal Protein S6 on 10 µg of PMA treated and untreated A431 cell lysates.
ターゲットの説明
~32 kDa observed
物理的形状
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
アナリシスノート
Control PMA treated and untreated A431 cell lysates
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
