06-229
Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody
Upstate®, from rabbit
別名:
CD340 antigen, MLN 19, NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2, c-erb B2/neu protein, neuroblastoma/glioblastoma derived oncogene homolog, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma der
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About This Item
UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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由来生物
rabbit
品質水準
抗体製品の状態
affinity purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
human, rat
包装
antibody small pack of 25 μL
メーカー／製品名
Upstate®
テクニック
immunocytochemistry: suitable
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
ターゲットの翻訳後修飾
phosphorylation (pTyr1248)
遺伝子情報
human ... ERBB2(2064)
関連するカテゴリー
詳細
The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.
特異性
Predicted to cross react with rat based on conservation of immunogenic sequence.
Recognizes and is specific for the activated form of erbB-2/HER-2 oncoprotein, MW ~138 kDa.
免疫原
Epitope: Tyr1248
Synthetic phosphopeptide, residues 1243-1255, corresponding to a major auto-phosphorylation (Tyr1248) site of human erbB-2/HER-2 (C-AENPEpYLGLDVPV).
アプリケーション
Research Category
細胞シグナル伝達
細胞シグナル伝達
Research Sub Category
成長因子及び受容体
キナーゼ及びホスファターゼ
成長因子及び受容体
キナーゼ及びホスファターゼ
Immunohistochemistry:
IHC was performed by an independent laboratory.
Immunocytochemistry:
2 µg/mL of a previous lot showed positive immunostaining for phospho-erbB2/HER-2 in EGF treated A431 cells.
IHC was performed by an independent laboratory.
Immunocytochemistry:
2 µg/mL of a previous lot showed positive immunostaining for phospho-erbB2/HER-2 in EGF treated A431 cells.
This Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody is validated for use in IC, WB for the detection of phospho-erbB-2/HER-2 (Tyr1248).
品質
Evaluated by Western Blot on A431+/-EGF lysates.
Western Blotting Analysis:
1:1000 dilution of this antibody detected phospho-erbB2/Her2 on 10 µg of A431+EGF lysates.
Western Blotting Analysis:
1:1000 dilution of this antibody detected phospho-erbB2/Her2 on 10 µg of A431+EGF lysates.
ターゲットの説明
185 kDa
物理的形状
ImmunoAffinity Purified
Double immunoaffinity purified rabbit polyclonal IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4
保管および安定性
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
アナリシスノート
Control
EGF treated A431 cells
EGF treated A431 cells
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
06-229:
06-229CA:
06-229-100UL:
06-229-25UL:
06-229BR:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Yuichi Abe et al.
Neuroscience research, 63(2), 138-148 (2008-12-17)
Ligands for the epidermal growth factor receptor ErbB1, such as epidermal growth factor (EGF) and transforming growth factor alpha (TGFalpha), negatively regulate synaptic maturation of GABAergic neurons in the developing neocortex. Here, we evaluated the effects of these ligands in
BIBX1382BS, but not AG1478 or PD153035, inhibits the ErbB kinases at different concentrations in intact cells.
M Egeblad, O H Mortensen, L C van Kempen, M Jaattela
Biochemical and biophysical research communications null
Activation of ErbB2 during wallerian degeneration of sciatic nerve.
Kwon, Y K, et al.
The Journal of Neuroscience, 17, 8293-8299 (1997)
Tonghui Wang et al.
Cancer science, 108(4), 671-677 (2017-02-07)
It is well documented that human epidermal growth factor receptor 2 (HER2) overexpression/amplification is associated with poor survival in breast cancer patients. However, it is largely unknown whether HER2 somatic mutations are associated with survival in HER2-negative breast cancer patients.
Bay846, a new irreversible small molecule inhibitor of EGFR and Her2, is highly effective against malignant brain tumor models.
Longo, Sharon L, et al.
Investigational New Drugs, 30, 2161-2172 (2012)
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