806013

APN-BCN

95%

• 別名: Bicyclo[6.1.0]non-4-yn-9-ylmethyl (4-(cyanoethynyl)phenyl)carbamate
• 実験式（ヒル表記法）: C₂₀H₁₈N₂O₂
• 分子量: 318.37
• UNSPSCコード: 12352200
• PubChem Substance ID: 329768983
• NACRES: NA.22

特性

• アッセイ: 95%
• 形状: powder
• 反応適合性: reagent type: cross-linking reagent
• 保管温度: −20°C
• SMILES記法: N#CC#CC1=CC=C(NC(OCC2C3CCC#CCCC23)=O)C=C1
• InChI: 1S/C19H16N2O2/c20-13-5-6-14-9-11-15(12-10-14)21-19(22)23-18-16-7-3-1-2-4-8-17(16)18/h9-12,16-18H,3-4,7-8H2,(H,21,22)
• InChI Key: LVFLVSUFJDGHRN-UHFFFAOYSA-N

詳細

アプリケーション

APN-BCN is a bifunctional crosslinker for thiol-to-azide coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions.

調製ノート

Standard protein labeling procedure (cysteine labeling).

• Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
• Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
• Incubate at room temperature for 2 hours.
• If necessary; purify the protein-BCN conjugate using size exclusion chromatography or ultrafiltration.
• The conjugate can be readily coupled with azide-containing molecules by incubating the components in an aqueous buffer.

*Note: avoid thiol-containing buffers.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H302
• 注意書き: P301 + P312 + P330
• 危険有害性の分類: Acute Tox. 4 Oral
• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable