806005

APN-Amine HCl

95%

• 別名: 3-(4-(4-(Aminomethyl)-1H-1,2,3-triazol-1-yl)phenyl)propiolonitrile hydrochloride
• 実験式（ヒル表記法）: C₁₂H₁₀N₅Cl
• 分子量: 259.69
• UNSPSCコード: 12352200
• PubChem Substance ID: 329768982

特性

• 品質水準: 100
• アッセイ: 95%
• フォーム: powder
• 反応適合性: reagent type: cross-linking reagent
• 保管温度: 2-8°C
• SMILES記法: N#CC#CC1=CC=C(N2C=C(CN)N=N2)C=C1.[H]Cl
• InChI: 1S/C12H9N5.ClH/c13-7-1-2-10-3-5-12(6-4-10)17-9-11(8-14)15-16-17;/h3-6,9H,8,14H2;1H
• InChI Key: JZGAUVVBWJHLGN-UHFFFAOYSA-N

詳細

アプリケーション

APN-Amine is a bifunctional crosslinker for thiol-to-carboxyl coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions. The amino group readily reacts with activated carboxylic acids (e.g. NHS-esters) in basic conditions to produce stable amide bonds. The APN group can be used for coupling with free thiols (e.g. labeling of free cysteines in proteins).

その他情報

Standard protein labeling procedure (cysteine labeling):

• Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
• Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
• Incubate at room temperature for 2 hours.
• If necessary, purify the protein conjugate using size exclusion chromatography or ultrafiltration.

*Note: avoid thiol-containing buffers.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable