803529

BMPH (N-(β-maleimidopropionic acid) hydrazide, trifluoroacetic acid salt)

• 実験式（ヒル表記法）: C₇H₉N₃O₃ · CF₃CO₂H
• 分子量: 297.19
• UNSPSCコード: 12352200
• PubChem Substance ID: 329768815
• NACRES: NA.22

特性

• アッセイ: ≥90%
• 形状: powder
• 分子量: 297.19
• 反応適合性: reagent type: cross-linking reagent
• 保管条件: desiccated
• 溶解性: water: soluble
• 官能基: hydrazide; maleimide
• 輸送温度: ambient
• 保管温度: 2-8°C
• SMILES記法: [NH3+]NC(CCN1C(C=CC1=O)=O)=O.[O-]C(C(F)(F)F)=O
• InChI: 1S/C7H9N3O3.C2HF3O2/c8-9-5(11)3-4-10-6(12)1-2-7(10)13;3-2(4,5)1(6)7/h1-2H,3-4,8H2,(H,9,11);(H,6,7)
• InChI Key: BADCXPKRBUEEMA-UHFFFAOYSA-N

詳細

詳細

BMPH, EMCH and KMUH are heterobifunctional crosslinkers containing sulfhydryl-reactive maleimide and carbonyl-reactive hydrazide moieties. Maleimides react with free sulfhydryls (-SH) to form stable thioether bonds. Hydrazide groups react with carbonyls (aldehydes and ketones) to form stable hydrazone bonds. Aldehyde groups can be created by periodate-oxidation of sialic acid and other sugar components of glycoprotein polysaccharides. Thus, these crosslinkers are useful for conjugating glycoproteins and sulfhydryl-containing peptides or proteins. Alternatively, the amine of the hydrazide moiety can be reacted to carboxyl groups using the crosslinker EDC.

特徴および利点

• Reactive groups: maleimide and hydrazide
• Reactive towards: sulfhydryl groups and carbonyl (aldehyde) groups
• Short (8.1A), sulfhydryl-to-aldehyde crosslinker with simple spacer arm (noncleavable)
• Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
• Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
• Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
• Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups

注意

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable