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E1131

Sigma-Aldrich

Exonuclease III from Escherichia coli BE25 /psGR3

buffered aqueous glycerol solution

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About This Item

CAS Number:
9037-44-9
Enzyme Commission number:
3.1.11.2 (BRENDA, IUBMB)
EC Number:
232-912-1
MDL number:
MFCD00163580
UNSPSC Code:
12352204

grade

Molecular Biology
for molecular biology

form

buffered aqueous glycerol solution

mol wt

28 kDa

UniProt accession no.

P09030

storage temp.

−20°C

Gene Information

Escherichia coli K12 ... xthA(946254)

General description

A 3′→5′ exonuclease which catalyzes the removal of mononucleotides from the 3′-end of dsDNA. The enzyme also has apurinic and apyrimidinic endonuclease, 3′-DNA phosphatase, and RNase H activities. Activity is strongly dependent on temperature, salt concentration, and the ratio of enzyme to DNA, therefore reaction conditions must be optimized for specific applications.

Application

Suitable for:
  • Production of strand specific probes
  • Preparation of single stranded templates for Sanger dideoxy sequencing
  • Site directed mutagenesis

Components

Exonuclease III is supplied as a solution in 5 mM potassium phosphate (pH 6.5), 200 mM KCl, 0.05 mM EDTA, 5 mM 2-mercaptoethanol, 200 μg/ml BSA, and 50% glycerol.

Unit Definition

One unit releases 1 nmole of acid-soluble nucleotides from sonicated calf thymus DNA in 30 min at 37 °C.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

E1131-5KU:4548173297804
E1131-2.5KU:
E1131-2.5KU-KC:
E1131-20KU:
E1131-BULK:
E1131-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
025K12621
085K6180
055K6026
015K2703
067H0796

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Jan Silhan et al.
Nucleic acids research, 40(5), 2065-2075 (2011-11-10)
We have previously demonstrated that the two Exonuclease III (Xth) family members present within the obligate human pathogen Neisseria meningitidis, NApe and NExo, are important for survival under conditions of oxidative stress. Of these, only NApe possesses AP endonuclease activity
Hui Chen et al.
Chemical communications (Cambridge, England), 48(2), 269-271 (2011-11-23)
We describe herein a novel exonuclease III aided amplification method based on single walled carbon nanotube quenching (EASQ) for sensitive and convenient nucleic acid detection, which enabled 80-fold decrease of detection limit for HIV1 DNA assay compared with no target
Xu-Hua Zhao et al.
Analytica chimica acta, 727, 67-70 (2012-05-01)
Based on the super fluorescence quenching efficiency of graphene oxide and exonuclease III aided signal amplification, we develop a facile, sensitive, rapid and cost-effective method for DNA detection. In the presence of target DNA, the target-probe hybridization forms a double-stranded
Lu Peng et al.
Biosensors & bioelectronics, 35(1), 475-478 (2012-04-03)
We have developed a novel DNA assay based on exonuclease III (ExoIII)-induced target recycling and the fluorescence quenching ability of graphene oxide (GO). This assay consists of a linear DNA probe labeled with a fluorophore in the middle. Introduction of
Feng Xuan et al.
Analytical chemistry, 84(12), 5216-5220 (2012-06-23)
Taking advantage of the preferential exodeoxyribonuclease activity of exonuclease III in combination with the difference in diffusivity between an oligonucleotide and a mononucleotide toward a negatively charged ITO electrode, a highly sensitive and selective electrochemical molecular beacon (eMB)-based DNA sensor
View All Related Papers

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