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E9034

Sigma-Aldrich

Phosphodiesterase 5A1 human

recombinant, expressed in Sf9 cells

Synonym(s):

3′,5′-cyclic-GMP phosphodiesterase, PDE 5A1

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About This Item

Enzyme Commission number:
3.1.4.25 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in Sf9 cells

Quality Level

200

form

solution

specific activity

≥2,000 unit/μg protein

mol wt

126 kDa

UniProt accession no.

O76074

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PDE5A(8654)

General description

Research area: Cell SignallingPDE5is located in various tissues such as the kidney, brain, lungs, cardiac myocytes,vascular smooth muscle cells, penile corpus cavernosum, platelets, andgastrointestinal tissues.

Biochem/physiol Actions

PDE5 regulates vascular smooth muscle contraction and is involved in NO-cGMP signaling in platelets to control aggregation. PDE5 may also be involved in the regulation of cGMP signaling in the brain and may modulate pressure-induced cardiac hypertrophy and fibrosis . Phosphodiesterase (PDE)is an enzyme that is used to break phosphodiester bonds. Phosphodiesterase 5 (PDE5), amolecular target for the treatment of erectile dysfunction and pulmonaryhypertension. PDE5 is used to find novel PDE5 inhibitors. PDE5 specifically targets cyclicguanosine monophosphate (cGMP) generated by the activation of nitric oxide (NO). PDE5 regulates cGMP signalingin the cardiovascular and other tissues. At the genetic level PDE5 isexpressed as various isoforms and undergoes phosphorylation as a post-translationalmodification that results in its activation. PDE5 is degraded through theubiquitin pathway due to its nitrosylation.

Physical properties

N-terminal GST-tagged 126 kDa full-length protein

Unit Definition

One unit will convert 1.0 picomole of 3′,5′-cGMP to 5′-GMP per minute at pH 7.4 at 37 °C.

Physical form

Supplied as a solution in 20% glycerol containing 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% TWEEN® 20 and 3 mM DTT

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Cartagena Act

Cartagena Act Listed

JAN Code

E9034-10UG-PW:
E9034-BULK:
E9034-10UG:
E9034-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Andrew T Bender et al.
Pharmacological reviews, 58(3), 488-520 (2006-09-14)
Cyclic nucleotide phosphodiesterases (PDEs) are enzymes that regulate the cellular levels of the second messengers, cAMP and cGMP, by controlling their rates of degradation. There are 11 different PDE families, with each family typically having several different isoforms and splice
Felicia Akuamoa et al.
Toxicology in vitro : an international journal published in association with BIBRA, 73, 105130-105130 (2021-03-05)
Herbal supplements sold as 'all natural' on various markets in Accra (Ghana) and advertised as highly efficacious in treating erectile dysfunction (ED) were bought and analysed by a PDE-5 enzyme inhibition assay. The claimed efficacy of these products could be
Wesam S Ahmed et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 134, 111128-111128 (2020-12-22)
Phosphodiesterase 5 (PDE5) is one of the most well-studied phosphodiesterases (PDEs) that specifically targets cGMP typically generated by nitric oxide (NO)-mediated activation of the soluble guanylyl cyclase. Given the crucial role of cGMP generated through the activation of this cellular

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