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AP0100

Sigma-Aldrich

Alkaline Phosphatase Diethanolamine Activity Kit

sufficient for 100 tests

Synonym(s):

ALP Activity Kit

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

usage

sufficient for 100 tests

Quality Level

200

storage temp.

2-8°C

General description

The Alkaline Phosphatase Diethanolamine Detection Kit provides ready-to-use reagents for detecting the presence of alkaline phosphatase activity. This simple assay to detect alkaline phosphatase activity uses p-Nitrophenyl Phosphate (pNPP) as the substrate.

Application

Alkaline Phosphatase Diethanolamine Activity Kit has been used:
  • in the detection of alkaline phosphatase (ALP) levels in the blood serum and osteoblasts
  • for monitoring ALP levels during osteoblast differentiation in mouse myoblast C2C12 cells
  • for determining ALP activity in the bone metastasis model of lung cancer
  • for measuring ALP levels in colon tissue homogenates

Suitability

Suitable for the measurement of alkaline phosphatase activity in a variety of samples

Principle

Alkaline phosphatase hydrolyzes pNPP to p-nitrophenol and inorganic phosphate. During incubation of the alkaline phosphatase sample and substrate at 37 C, the reaction is followed by monitoring the increase in absorbance at 405 nm.

Kit Components Also Available Separately

Product No.
Description
SDS
  • Alkaline Phosphatase Diethanolamine Reaction Buffer
  • P4744Phosphatase substrate, powderSDS
  • P6774Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous solution, ≥2,000 DEA units/mg proteinSDS

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H315,H318,H361fd,H373

Hazard Classifications

Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

Target Organs

Kidney,Liver,Blood

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

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FSL

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ISHL Indicated Name

Please refer to KIT Component information

ISHL Notified Names

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Cartagena Act

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JAN Code

キットコンポーネントの情報を参照してください

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Certificates of Analysis (COA)

Lot/Batch Number
SLCQ1301
SLCM3820
SLCM3819
SLCM9477
SLCH1704

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