For testing purposes, this product is prepared in cold deionized water. While PBS has not been tested as a solvent, the enzyme is expected to be soluble. Please see the link below to review the assay protocol for cellulase and hemicellulase:
https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-cellulase
C1794
Cellulase from Trichoderma sp.
Cellulase from Trichoderma sp.
BioReagent, suitable for plant cell culture, 3-10 units/mg solid
Synonym(s):
β-1,4-glucanase
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5000 UNITS
¥26,040
10000 UNITS
¥43,330
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5000 UNITS
¥26,040
10000 UNITS
¥43,330
About This Item
Recommended Products
biological source
fungus (Trichoderma sp.)
Quality Level
product line
BioReagent
form
powder
specific activity
3-10 units/mg solid
contains
maltodextrin
greener alternative product characteristics
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
cell culture | plant: suitable
application(s)
agriculture
greener alternative category
, Enabling
storage temp.
2-8°C
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General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.
Application
Cellulase (β-1,4-glucanase) is a natural enzyme produced by the filamentous fungi Trichoderma viride which promotes the endohydrolysis of (1→4)-beta-D-glucosidic linkages in cellulose and lichenin. Cellulases are important for processing potential commercial products such as cellulosic ethanol.
Unit Definition
One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).
substrate
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
C1794-5KU:
C1794-VAR:
C1794-BULK:
C1794-10KU:
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Jin Zhou et al.
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To improve the enzymatic hydrolytic efficiency and reduce production cost, a statistically designed experimental approach was used to optimize the composition of cellulase mixture so as to maximize the amount of glucose produced from steam-exploded corn stover (SECS). Using seven
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Alamethicin is a membrane-active peptide isolated from the beneficial root-colonising fungus Trichoderma viride. This peptide can insert into membranes to form voltage-dependent pores. We have previously shown that alamethicin efficiently permeabilises the plasma membrane, mitochondria and plastids of cultured plant
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Protocols
To standardize an enzymatic assay procedure of cellulase.
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Hi, I was wondering how to prepare the enzyme solution? Can I just dilute it on PBS? Thanks
1 answer-
Helpful?
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Hello, what is the Uniprot number or Gene ID for C1794-5KU? Thank you
1 answer-
The Gene ID for Cellulase from Trichoderma sp. (AKA Gliocladium sp.) is GH45. Please see information at the links below:
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